Back to Search
Start Over
A novel short neurotoxin, cobrotoxin c, from monocellate cobra (Naja kaouthia) venom: isolation and purification, primary and secondary structure determination, and tertiary structure modeling.
- Source :
-
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP [Comp Biochem Physiol C Toxicol Pharmacol] 2002 May; Vol. 132 (1), pp. 113-21. - Publication Year :
- 2002
-
Abstract
- A novel short neurotoxin, cobrotoxin c (CBT C) was isolated from the venom of monocellate cobra (Naja kaouthia) using a combination of ion-exchange chromatography and FPLC. Its primary structure was determined by Edman degradation. CBT C is composed of 61 amino acid residues. It differs from cobrotoxin b (CBT B) by only two amino acid substitutions, Thr/Ala11 and Arg/Thr56, which are not located on the functionally important regions by sequence similarity. However, the LD50 is 0.08 mg/g to mice, i.e. approximately five-fold higher than for CBT B. Strikingly, a structure-function relationship analysis suggests the existence of a functionally important domain on the outside of Loop III of CBT C. The functionally important basic residues on the outside of Loop III might have a pairwise interaction with alpha subunit, instead of gamma or delta subunits of the nicotinic acetylcholine receptor (nAChR).
- Subjects :
- Amino Acid Sequence
Animals
Biological Assay
Chromatography, High Pressure Liquid
Circular Dichroism
Cobra Neurotoxin Proteins toxicity
Elapidae
Lethal Dose 50
Models, Molecular
Molecular Sequence Data
Protein Structure, Secondary
Protein Structure, Tertiary
Sequence Homology
Structure-Activity Relationship
Cobra Neurotoxin Proteins chemistry
Cobra Neurotoxin Proteins isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1532-0456
- Volume :
- 132
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
- Publication Type :
- Academic Journal
- Accession number :
- 12039691
- Full Text :
- https://doi.org/10.1016/s1532-0456(02)00049-2