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A mouse model of inflammatory root resorption induced by pulpal infection.
- Source :
-
Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics [Oral Surg Oral Med Oral Pathol Oral Radiol Endod] 2002 Apr; Vol. 93 (4), pp. 461-8. - Publication Year :
- 2002
-
Abstract
- Objective: The present study was undertaken to determine the frequency and extent of apical root resorption associated with induced periradicular lesions in mice.<br />Study Design: Bone and root resorption was quantified by using two- and three-dimensional micro-computed tomography (mu-CT) in the lower first molars of mice subjected to pulp exposure and infection.<br />Results: mu-CT measurements showed significant apical resorption in exposed and infected teeth, resulting in an average distal root shortening of 12.7% (P <.001 vs unexposed). These findings were confirmed with three-dimensional reconstituted images that showed thinning and shortening of the distal root. Tartrate-resistant acid phosphatase clastic cells were associated with resorption lacunae on the cementum of root apices, as well as on bone at the periphery of the periradicular lesions. Brown and Brenn staining showed the presence of bacteria in dentinal tubules adjacent to resorbed cementum.<br />Conclusions: Apical root resorption is a prominent and consistent finding associated with periradicular infection in the mouse. This species represents a convenient model for studying the pathogenesis of inflammatory root resorption in vivo.
- Subjects :
- Acid Phosphatase analysis
Animals
Biomarkers analysis
Bone Resorption diagnostic imaging
Bone Resorption etiology
Bone Resorption pathology
Coloring Agents
Dental Cementum microbiology
Dental Cementum pathology
Dental Pulp Diseases microbiology
Dental Pulp Exposure complications
Dental Pulp Exposure microbiology
Dentin microbiology
Disease Models, Animal
Gram-Negative Bacteria isolation & purification
Image Processing, Computer-Assisted methods
Imaging, Three-Dimensional
Isoenzymes analysis
Male
Mandibular Diseases diagnostic imaging
Mandibular Diseases etiology
Mandibular Diseases pathology
Mice
Mice, Inbred C57BL
Mice, Inbred Strains
Molar
Periapical Diseases diagnostic imaging
Periapical Diseases etiology
Regression Analysis
Root Resorption diagnostic imaging
Root Resorption pathology
Statistics as Topic
Tartrate-Resistant Acid Phosphatase
Tomography, X-Ray Computed methods
Tooth Apex diagnostic imaging
Tooth Apex pathology
Tooth Root diagnostic imaging
Dental Pulp Diseases complications
Root Resorption etiology
Subjects
Details
- Language :
- English
- ISSN :
- 1079-2104
- Volume :
- 93
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics
- Publication Type :
- Academic Journal
- Accession number :
- 12029286
- Full Text :
- https://doi.org/10.1067/moe.2002.122641