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Differential and competitive activation of human immune cells by distinct classes of CpG oligodeoxynucleotide.
- Source :
-
Journal of leukocyte biology [J Leukoc Biol] 2002 May; Vol. 71 (5), pp. 813-20. - Publication Year :
- 2002
-
Abstract
- Synthetic oligodeoxynucleotides (ODN) expressing "CpG motifs" show promise as immune adjuvants, antiallergens, anticancer, and immunoprotective agents. Two structurally distinct classes of CpG ODN have been identified that stimulate human PBMC. This work establishes that both types of ODN bind to and are internalized by the same individual B cells, NK cells, and monocytes. However, the intracellular localization of "D" and "K" ODN differs, as does their functional activity: "K" type ODN trigger monocytes and B cells to proliferate and secrete IL-6 and IgM, whereas "D" type ODN induce NK cells to produce IFN-gamma and monocytes to differentiate into CD83(+)/CD86(+) dendritic cells. In monocytes, these two types of ODN (which differ in backbone composition and CpG motif) cross-inhibit one another's activity. Thus, different types of CpG ODN have distinct and in some cases incompatible effects on the same cells, a finding with important implications for the therapeutic use of these agents.
- Subjects :
- B-Lymphocytes drug effects
B-Lymphocytes immunology
Cells, Cultured
Endocytosis
Humans
Killer Cells, Natural drug effects
Killer Cells, Natural immunology
Leukocytes, Mononuclear immunology
Leukocytes, Mononuclear metabolism
Lymphocyte Activation
Monocytes drug effects
Monocytes immunology
Oligodeoxyribonucleotides classification
Oligodeoxyribonucleotides metabolism
Adjuvants, Immunologic pharmacology
Leukocytes, Mononuclear drug effects
Oligodeoxyribonucleotides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0741-5400
- Volume :
- 71
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of leukocyte biology
- Publication Type :
- Academic Journal
- Accession number :
- 11994506