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Altered expression of vascular natriuretic peptide receptors in experimental hypertensive rats.
- Source :
-
Clinical and experimental pharmacology & physiology [Clin Exp Pharmacol Physiol] 2002 Apr; Vol. 29 (4), pp. 299-303. - Publication Year :
- 2002
-
Abstract
- 1. The aim of the present study was to determine whether the regulation of vascular natriuretic peptide receptors (NPR) is related to the local renin-angiotensin system (RAS). 2. Male Sprague-Dawley rats were made two-kidney, one-clip (2K1C) and deoxycorticosterone acetate (DOCA)-salt hypertensive to activate and inhibit the RAS, respectively. Another model of hypertension was induced by treatment with an inhibitor of nitric oxide synthesis, namely NG-nitro-L-arginine methyl ester (L-NAME). 3. The mRNA expression of NPR-A, NPR-C, angiotensin- converting enzyme (ACE) and angiotensin AT1 receptors was determined in the thoracic aorta by semiquantitative reverse transcription-polymerase chain reaction. The particulate guanylyl cyclase activity stimulated by atrial natriuretic peptide (ANP) was also determined in the membrane fraction of the thoracic aorta. 4. The plasma concentrations of ANP were increased significantly in the three models of hypertension. Plasma renin activity was increased in 2K1C hypertension, decreased in DOCA-salt hypertension and not significantly altered in L-NAME hypertension. 5. The mRNA expression of NPR-A and NPR-C was decreased, whereas that of ACE and AT1 receptors was increased in 2K1C and L-NAME hypertension. The mRNA expression of NPR-A and NPR-C was increased, whereas that of ACE and AT1 receptors was decreased in DOCA-salt hypertension. 6. The particulate guanylyl cyclase activity was decreased in 2K1C and L-NAME hypertension and increased in DOCA-salt hypertension. 7. The vascular expression of NPR may be reciprocally regulated by local RAS activity.
- Subjects :
- Animals
Aorta, Thoracic
Enzyme Activation
Guanylate Cyclase blood
Guanylate Cyclase genetics
Guanylate Cyclase metabolism
Hypertension chemically induced
Male
Peptidyl-Dipeptidase A biosynthesis
Peptidyl-Dipeptidase A genetics
RNA, Messenger biosynthesis
Rats
Rats, Sprague-Dawley
Receptor, Angiotensin, Type 1
Receptors, Angiotensin biosynthesis
Receptors, Angiotensin genetics
Receptors, Atrial Natriuretic Factor blood
Receptors, Atrial Natriuretic Factor genetics
Renin blood
Renin-Angiotensin System physiology
Guanylate Cyclase biosynthesis
Hypertension metabolism
Receptors, Atrial Natriuretic Factor biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0305-1870
- Volume :
- 29
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Clinical and experimental pharmacology & physiology
- Publication Type :
- Academic Journal
- Accession number :
- 11985539
- Full Text :
- https://doi.org/10.1046/j.1440-1681.2002.03647.x