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Predominance of caecal injury in a new dextran sulphate sodium treatment in rats: histopathological and fermentative characteristics.
- Source :
-
European journal of gastroenterology & hepatology [Eur J Gastroenterol Hepatol] 2002 May; Vol. 14 (5), pp. 535-42. - Publication Year :
- 2002
-
Abstract
- Objectives: Cyclic administrations of dextran sulphate sodium (DSS) alternating with distilled water usually induce chronic colitis after a few weeks. In order to obtain stable chronic colitis (without recovery or relapse) in a few days, a new continuous DSS treatment was tested and characterized. Short-chain fatty acids (SCFAs), which remain poorly documented in experimental colitis, were also investigated.<br />Methods: Thirty-six Sprague-Dawley rats were treated with 5% DSS for 7 days (DI) followed by 3% DSS for 7 days (DM) or 14 days (DF). Control rats received only water. Inflammatory injuries in the caecum and the colon were assessed by macroscopic (colon length, caecum weight, damages score) and histological parameters. SCFAs (acetate, propionate, butyrate) were quantified individually in caecal, proximal and distal contents.<br />Results: Macroscopic and histological observations revealed that this continuous DSS treatment induced acute inflammation (DI) followed rapidly by chronic active colitis. The latter was uncommonly predominant in the caecum and the distal colon, and was also associated with some fermentative disturbances. Caecal SCFA concentrations decreased with DSS at DI and DM. The molar ratio of caecal butyrate increased with DSS. Acetate decreased in the colon while propionate increased.<br />Conclusion: This new DSS treatment is able to induce in a few days stable chronic inflammation with caecal and distal predominant injuries, and mild fermentative caeco-colonic alterations. This model could contribute to the study of potential anti-inflammatory effects of prebiotics.
- Subjects :
- Animals
Cecal Diseases metabolism
Cecum metabolism
Cecum pathology
Colitis metabolism
Colon metabolism
Colon pathology
Fatty Acids, Volatile metabolism
Fermentation
Inflammation
Male
Rats
Rats, Sprague-Dawley
Weight Gain
Cecal Diseases chemically induced
Colitis chemically induced
Dextran Sulfate toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 0954-691X
- Volume :
- 14
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- European journal of gastroenterology & hepatology
- Publication Type :
- Academic Journal
- Accession number :
- 11984152
- Full Text :
- https://doi.org/10.1097/00042737-200205000-00011