Back to Search
Start Over
Nitric oxide of human colorectal adenocarcinoma cell lines promotes tumour cell invasion.
- Source :
-
British journal of cancer [Br J Cancer] 2002 Apr 22; Vol. 86 (8), pp. 1310-5. - Publication Year :
- 2002
-
Abstract
- The present study investigates the role of nitric oxide and the involvement of nitric oxide synthase II isoform on the invasion of human colorectal adenocarcinoma cell lines HRT-18 and HT-29. HRT-18 cells, which constitutively express nitric oxide synthase II mRNA were three-fold more invasive in a Matrigel invasion assay than nitric oxide synthase II mRNA negative HT-29 cells. Treatment of HT-29 cells with the nitric oxide donor Deta NONOate (50 nM) as well as induction of nitric oxide synthase II mRNA and production of endogenous nitric oxide by inflammatory cytokines (IFN-gamma and IL-1alpha) increased the invasiveness of HT-29 cells by approximately 40% and 75%, respectively. In HT-29 cells nitric oxide synthase II mRNA was also induced in co-culture with human monocytes. The invasiveness of HRT-18 cells and stimulated HT-29 cells was partly inhibited by the nitric oxide synthase II inhibitor 1400 W. These results show that nitric oxide increases the invasion of human colorectal adenocarcinoma cell lines HRT-18 and HT-29, and the involvement of nitric oxide synthase II isoform in tumour cell invasion. Therefore, the production of nitric oxide and secretion of pro-inflammatory cytokines by tumour-associated macrophages, which in turn induce nitric oxide synthase II isoform in tumour cells, promotes tumour cell invasiveness.<br /> (Copyright 2002 Cancer Research UK)
- Subjects :
- Adenocarcinoma enzymology
Adenocarcinoma genetics
Amidines pharmacology
Benzylamines pharmacology
Cell Division drug effects
Coculture Techniques
Colorectal Neoplasms enzymology
Colorectal Neoplasms genetics
Gene Expression Regulation, Neoplastic drug effects
Humans
Interferon-gamma pharmacology
Interleukin-1 pharmacology
Monocytes
Neoplasm Invasiveness
Nitric Oxide Synthase antagonists & inhibitors
Nitric Oxide Synthase genetics
Nitric Oxide Synthase metabolism
Nitric Oxide Synthase Type II
Nitroso Compounds pharmacology
RNA, Messenger genetics
RNA, Messenger metabolism
RNA, Neoplasm genetics
RNA, Neoplasm metabolism
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
Adenocarcinoma metabolism
Adenocarcinoma pathology
Colorectal Neoplasms metabolism
Colorectal Neoplasms pathology
Nitric Oxide metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0007-0920
- Volume :
- 86
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- British journal of cancer
- Publication Type :
- Academic Journal
- Accession number :
- 11953890
- Full Text :
- https://doi.org/10.1038/sj.bjc.6600224