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[Study of the role of calcitonin during the embryo implantation].
- Source :
-
Zhonghua fu chan ke za zhi [Zhonghua Fu Chan Ke Za Zhi] 2002 Jan; Vol. 37 (1), pp. 16-8. - Publication Year :
- 2002
-
Abstract
- Objective: To observe the role of calcitonin (CT) during the implantation.<br />Methods: Human endometrial epithelial cells were cultured. After stimulated with various concentrations of CT, intracellular calcium (Ca(2+)) in the epithelial and stroma cells and pre-embryos were measured by the laser scanning confocal microscope.<br />Results: When stimulated with different concentrations of CT, mean fluorescence levels in the epithelial cells were similar to that of the control. However CT can improve intracellar Ca(2+) of preimplantation embryos and stroma cells in a dose-dependent manner and significantly higher than those of controls. When 10 nmol/L CT was added to the culture medium, the intracellular Ca(2+) concentration in 8-cell embryos rose immediately. Embryo exposure to CT was followed by a series of Ca(2+) bursts that persisted for at least 2 hours. No change in Ca(2+) was observed when culture medium alone was added to the embryos. Pre-loading embryos and stoma cells with the Ca(2+) chelator, prevented the increased fluorescence after CT addition.<br />Conclusions: CT play an important role during the procesess of implantation. It maybe improve intracellar of preembryos and accerate the development of preembryos.
- Subjects :
- Blastomeres drug effects
Blastomeres physiology
Embryo Implantation physiology
Endometrium drug effects
Endometrium physiology
Epithelial Cells drug effects
Epithelial Cells physiology
Female
Humans
In Vitro Techniques
Male
Pregnancy
Stromal Cells drug effects
Stromal Cells physiology
Calcitonin pharmacology
Calcium metabolism
Embryo Implantation drug effects
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0529-567X
- Volume :
- 37
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Zhonghua fu chan ke za zhi
- Publication Type :
- Academic Journal
- Accession number :
- 11953057