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LIS1, CLIP-170's key to the dynein/dynactin pathway.
- Source :
-
Molecular and cellular biology [Mol Cell Biol] 2002 May; Vol. 22 (9), pp. 3089-102. - Publication Year :
- 2002
-
Abstract
- CLIP-170 is a plus-end tracking protein which may act as an anticatastrophe factor. It has been proposed to mediate the association of dynein/dynactin to microtubule (MT) plus ends, and it also binds to kinetochores in a dynein/dynactin-dependent fashion, both via its C-terminal domain. This domain contains two zinc finger motifs (proximal and distal), which are hypothesized to mediate protein-protein interactions. LIS1, a protein implicated in brain development, acts in several processes mediated by the dynein/dynactin pathway by interacting with dynein and other proteins. Here we demonstrate colocalization and direct interaction between CLIP-170 and LIS1. In mammalian cells, LIS1 recruitment to kinetochores is dynein/dynactin dependent, and recruitment there of CLIP-170 is dependent on its site of binding to LIS1, located in the distal zinc finger motif. Overexpression of CLIP-170 results in a zinc finger-dependent localization of a phospho-LIS1 isoform and dynactin to MT bundles, raising the possibility that CLIP-170 and LIS1 regulate dynein/dynactin binding to MTs. This work suggests that LIS1 is a regulated adapter between CLIP-170 and cytoplasmic dynein at sites involved in cargo-MT loading, and/or in the control of MT dynamics.
- Subjects :
- 1-Alkyl-2-acetylglycerophosphocholine Esterase
Animals
COS Cells
Dynactin Complex
HeLa Cells
Humans
Interphase
Kinetochores metabolism
Microscopy, Fluorescence
Microtubules metabolism
Neoplasm Proteins
Protein Binding
Protein Isoforms metabolism
Protein Structure, Tertiary
Signal Transduction
Zinc Fingers
Dyneins metabolism
Microtubule-Associated Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0270-7306
- Volume :
- 22
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Molecular and cellular biology
- Publication Type :
- Academic Journal
- Accession number :
- 11940666
- Full Text :
- https://doi.org/10.1128/MCB.22.9.3089-3102.2002