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Deletions are easy detectable in cochlear mitochondrial DNA of Cu/Zn superoxide dismutase gene knockout mice.
- Source :
-
Chinese medical journal [Chin Med J (Engl)] 2002 Feb; Vol. 115 (2), pp. 258-63. - Publication Year :
- 2002
-
Abstract
- Objectives: To investigate the tissue specificity of reactive oxygen species (ROS) damage to mitochondrial DNA (mtDNA) and to determine whether cochlear mtDNA is a sensitive target for ROS damage.<br />Methods: 10 Cu/ZnSOD gene (Cu/Zn superoxide dismutase gene, Sod1) knockout mice and 16 wild-type mice were analyzed by nested polymerase chain reaction (PCR).<br />Results: Three deletions were detected in various tissues of Sod1 knockout mice. MtDNA3867bp and mtDNA3726bp deletions were the most visible, and mtDNA4236bp deletion was barely detected in these tissues. There were obvious differences in the ratio of deleted mtDNA/total mtDNA in different tissue. Deleted mtDNA was most abundant in the liver and kidney and less in cochlea, heart and brain. The lowest was in spleen and skin. The ratio in various tissues was 3 - 20 times in Sod1 knockout mice over wild-type mice. In cochlea, the ratio was about 15.<br />Conclusions: Without the protection of Sod1, ROS can lead to mtDNA deletions in various tissues with significant tissue specificity. Cochlear mtDNA is a sensitive target for ROS damage.
- Subjects :
- Animals
Base Sequence
Brain metabolism
DNA Mutational Analysis
DNA, Mitochondrial chemistry
Kidney metabolism
Liver metabolism
Mice
Mice, Inbred Strains
Mice, Knockout
Molecular Sequence Data
Myocardium metabolism
RNA, Ribosomal genetics
Sequence Deletion
Skin metabolism
Spleen metabolism
Cochlea metabolism
DNA, Mitochondrial genetics
Superoxide Dismutase genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0366-6999
- Volume :
- 115
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Chinese medical journal
- Publication Type :
- Academic Journal
- Accession number :
- 11940344