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Conserved structure for single-stranded telomeric DNA recognition.
- Source :
-
Science (New York, N.Y.) [Science] 2002 Apr 05; Vol. 296 (5565), pp. 145-7. - Publication Year :
- 2002
-
Abstract
- The essential Cdc13 protein in the yeast Saccharomyces cerevisiae is a single-stranded telomeric DNA binding protein required for chromosome end protection and telomere replication. Here we report the solution structure of the Cdc13 DNA binding domain in complex with telomeric DNA. The structure reveals the use of a single OB (oligonucleotide/oligosaccharide binding) fold augmented by an unusually large loop for DNA recognition. This OB fold is structurally similar to OB folds found in the ciliated protozoan telomere end-binding protein, although no sequence similarity is apparent between them. The common usage of an OB fold for telomeric DNA interaction demonstrates conservation of end-protection mechanisms among eukaryotes.
- Subjects :
- Binding Sites
DNA, Fungal chemistry
DNA, Single-Stranded chemistry
DNA-Binding Proteins metabolism
Ligands
Models, Molecular
Nuclear Magnetic Resonance, Biomolecular
Protein Binding
Protein Conformation
Protein Folding
Protein Structure, Secondary
Protein Structure, Tertiary
Saccharomyces cerevisiae Proteins metabolism
DNA, Fungal metabolism
DNA, Single-Stranded metabolism
DNA-Binding Proteins chemistry
Saccharomyces cerevisiae Proteins chemistry
Telomere metabolism
Telomere-Binding Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9203
- Volume :
- 296
- Issue :
- 5565
- Database :
- MEDLINE
- Journal :
- Science (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 11935027
- Full Text :
- https://doi.org/10.1126/science.1068799