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D-AP5 blocks the increase of intracellular free Ca2+ induced by glutamate in isolated cochlear IHCs.
- Source :
-
Chinese medical journal [Chin Med J (Engl)] 2002 Jan; Vol. 115 (1), pp. 89-93. - Publication Year :
- 2002
-
Abstract
- Objective: To investigate the effect of D-AP5 (D-2-amino-5-phosphonopentanoate, a specific NMDA-antagonist) on the increase of intracellular free Ca2+ concentration ([Ca2+]i) induced by glutamate in isolated cochlear inner hair cells (IHCs), and to detect the autoreceptors of the IHC membrane.<br />Methods: When a laser scanning confocal microscope (LSCM) was used, the exogenous glutamate (Glu)-induced changes in [Ca2+]i of isolated IHCs and OHCs of guinea pig cochlea were observed with fluo-3, a fluorescent probe for [Ca2+]i. After D-AP5 or CNQX (6--cyano--7--nitroguinoxaline--2, 3--dione, a specific AMPA- antagonist) was administered, the exogenous glutamate (Glu)-induced changes in [Ca2+]i of isolated IHCs were recorded.<br />Results: In the presence of a low concentration Glu (3.85 mumol/L), there was an increase of [Ca2+]i in IHCs, whereas there was no change in OHCs. When 50 mumol/L of D-AP5 was administrated in advance, Glu did not induce a corresponding increase in [Ca2+]i in IHCs, and 50 mumol/L of CNQX did not completely block the increase of [Ca2+]i in IHCs.<br />Conclusions: These results suggest that the autoreceptors existing in the IHC membrane are mainly of NMDA type, while there are relatively few AMPA receptors. Exogenous Glu is capable of increasing [Ca2+]i in IHCs by acting on the NMDA autoreceptor of IHCs in a positive feedback manner.
- Subjects :
- 6-Cyano-7-nitroquinoxaline-2,3-dione pharmacology
Animals
Guinea Pigs
Hair Cells, Auditory, Inner metabolism
In Vitro Techniques
2-Amino-5-phosphonovalerate pharmacology
Calcium metabolism
Excitatory Amino Acid Antagonists pharmacology
Glutamic Acid pharmacology
Hair Cells, Auditory, Inner drug effects
Receptors, N-Methyl-D-Aspartate antagonists & inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 0366-6999
- Volume :
- 115
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Chinese medical journal
- Publication Type :
- Academic Journal
- Accession number :
- 11930669