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Analysis of the GM-CSF and GM-CSF/IL-3/IL-5 receptor common beta chain in a patient with pulmonary alveolar proteinosis.
- Source :
-
Chinese medical journal [Chin Med J (Engl)] 2002 Jan; Vol. 115 (1), pp. 76-80. - Publication Year :
- 2002
-
Abstract
- Objective: To investigate the expression of the granulocyte-macrophage colony-stimulating factor (GM-CSF) and GM-CSF/IL-3/IL-5 receptor common beta chain (beta c receptor) in an adult patient with idiopathic pulmonary alveolar proteinosis (PAP), so as to demonstrate the possible association of the GM-CSF and beta c receptor with the pathogenesis of human PAP.<br />Methods: The GM-CSF levels were measured with a commercial ELISA kit (sensitivity 5 pg/ml) and the beta c receptor expression on the cell surface was detected by flow cytometry analysis. Reverse transcription polymerase chain reaction (RT-PCR) analysis was employed to detect the expression of the GM-CSF mRNA and the beta c receptor mRNA in peripheral blood mononuclear cells and alveolar macrophages. The entire coding regions of the GM-CSF cDNA and the beta c receptor cDNA were sequenced by the Sanger dideoxy-mediated chain termination method to detect possible mutations.<br />Results: The patient with PAP failed to release the GM-CSF protein either from circulating mononuclear cells or from alveolar macrophages. The expression of the GM-CSF mRNA was normal after the stimulation of lipopolysaccharide, whereas a point mutation at position 382 of the GM-CSF cDNA from "T" to "C" was revealed by cDNA sequencing, which caused a change in amino acid 117 of the protein from isoleucine to threonine. The beta c receptor expression on the cell surface was normal, and the beta c receptor mRNA expression and the sequence of the entire coding region of the beta c receptor were also normal.<br />Conclusions: The decreased GM-CSF production is associated with the pathogenesis of human PAP. A point mutation of the GM-CSF cDNA may contribute to the decreased GM-CSF production in our adult PAP patient. The mutation of the beta c receptor in some of paediatric patients with PAP may not be a common problem in adult patients.
- Subjects :
- DNA, Complementary chemistry
Granulocyte-Macrophage Colony-Stimulating Factor analysis
Humans
Male
Middle Aged
Pulmonary Alveolar Proteinosis etiology
RNA, Messenger analysis
Receptors, Cytokine genetics
Granulocyte-Macrophage Colony-Stimulating Factor biosynthesis
Pulmonary Alveolar Proteinosis metabolism
Receptors, Cytokine biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0366-6999
- Volume :
- 115
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Chinese medical journal
- Publication Type :
- Academic Journal
- Accession number :
- 11930665
- Full Text :
- https://doi.org/10.3901/jme.2002.supp.076