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Evidence for differential expression of candida albicans virulence genes during oral infection in intact and human immunodeficiency virus type 1-transgenic mice.
- Source :
-
The Journal of infectious diseases [J Infect Dis] 2002 Apr 15; Vol. 185 (8), pp. 1094-102. Date of Electronic Publication: 2002 Mar 21. - Publication Year :
- 2002
-
Abstract
- To comprehensively assess the in vivo expression of Candida albicans hydrolytic enzyme genes during oropharyngeal candidiasis (OPC), a controlled sequential analysis of the temporal expression of individual members of the SAP (secretory aspartyl proteinase) gene family and PLB1 (phospholipase B) in a murine model of OPC was conducted. Acute infections in intact C3H and DBA/2 mice were terminated by clearance of C. albicans within 7 days after oral inoculation, but transgenic (Tg) mice expressing human immunodeficiency virus type 1 were persistently colonized until a final outgrowth before death. In contrast to the sustained expression of other SAP genes and PLB1, SAP7 and SAP8 were conspicuously distinguished by their transient expression in both intact and Tg mice. SAP5 and SAP9 were most strongly expressed throughout the course of infection in the Tg mice. These findings indicate that expression of individual members of the C. albicans SAP gene family is differentially regulated during experimental OPC.
- Subjects :
- Animals
Aspartic Acid Endopeptidases genetics
Candida albicans pathogenicity
Candidiasis, Oral virology
Disease Models, Animal
Female
Fungal Proteins genetics
Male
Mice
Mice, Inbred C3H
Mice, Inbred DBA
Mice, Transgenic
Reproducibility of Results
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
Candida albicans genetics
Candidiasis, Oral etiology
Gene Expression Regulation, Fungal
HIV-1 physiology
Virulence genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1899
- Volume :
- 185
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- The Journal of infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 11930319
- Full Text :
- https://doi.org/10.1086/340035