Back to Search
Start Over
Detection and multigenic characterization of a herpesvirus associated with malignant catarrhal fever in white-tailed deer (Odocoileus virginianus) from Missouri.
- Source :
-
Journal of clinical microbiology [J Clin Microbiol] 2002 Apr; Vol. 40 (4), pp. 1311-8. - Publication Year :
- 2002
-
Abstract
- Between 1998 and 2001, tissues from four captive white-tailed deer were observed to have histologic lesions of systemic lymphocytic vasculitis. These lesions suggested malignant catarrhal fever, although epizootic hemorrhagic disease and bluetongue were included in the differential diagnosis. Initial diagnostic efforts, including virus isolation and reverse transcription-PCR for epizootic hemorrhagic disease virus and bluetongue virus, failed to identify an etiologic agent. However, consensus primer PCR targeted to the herpesvirus DNA polymerase gene detected viral genomic DNA in each of these four cases. Nucleotide sequence analysis of the amplified product demonstrated that the detected virus was identical over the compared region to the recently described malignant catarrhal fever virus of white-tailed deer (H. Li, N. Dyer, J. Keller, and T. B. Crawford, J. Clin. Microbiol. 38:1313-1318, 2000). Additional nucleotide sequencing of both the DNA polymerase gene and DNA packaging gene followed by phylogenetic analysis solidified this newly recognized herpesvirus as a member of the Gammaherpesvirinae and suggests that this virus, along with ovine herpesvirus 2, alcelaphine herpesvirus 1, alcelaphine herpesvirus 2 and caprine herpesvirus 2, may be part of a separate clade within this subfamily.
- Subjects :
- Animals
Base Sequence
Gammaherpesvirinae genetics
Gammaherpesvirinae isolation & purification
Herpesviridae Infections pathology
Herpesviridae Infections virology
Malignant Catarrh pathology
Missouri
Molecular Sequence Data
Phylogeny
Sequence Analysis, DNA
Bacterial Proteins genetics
DNA-Directed DNA Polymerase genetics
Deer
Gammaherpesvirinae classification
Malignant Catarrh virology
Subjects
Details
- Language :
- English
- ISSN :
- 0095-1137
- Volume :
- 40
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of clinical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 11923350
- Full Text :
- https://doi.org/10.1128/JCM.40.4.1311-1318.2002