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Evidence for a dimeric structure of rat liver serine dehydratase.
- Source :
-
The international journal of biochemistry & cell biology [Int J Biochem Cell Biol] 2002 May; Vol. 34 (5), pp. 533-43. - Publication Year :
- 2002
-
Abstract
- Rat liver serine dehydratase (SDH) is known to be involved in gluconeogenesis. It has long been believed to be a dimeric protein with the subunit molecular weight (M(r)) of 34,000. Recently, sheep liver SDH was reported to be a monomer with a M(r) of 38,000. The native M(r) of rat SDH was only determined by the ultracentrifugation method more than three decades ago, and that of sheep SDH was done by the method of gel chromatography. The primary to quaternary structures of a given enzyme in a specific mammalian organ are usually conserved among various species. The aim of the present investigation is to clarify the structural differences between rat and sheep SDHs. First, we found that the amino acid composition reported for sheep SDH was statistically similar to that of rat SDH. Second, immunoblot analysis using anti-rat SDH IgG as the probe showed the size of sheep SDH to be a M(r) of 30,500, whereas that of SDH was about M(r) of 35,000. On the other hand, the native size of rat SDH was assessed by two methods: (1) the laser light scattering method demonstrated that rat SDH had a M(r) of 66,800, consistent with the previous value (M(r)=64,000); (2) cross-linking experiments of the purified rat SDH with dimethyl suberimidate revealed the existence of a dimeric form by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The present results clearly confirm that rat SDH is a dimer, and suggest that sheep SDH is similar to rat SDH immunologically, but with a molecular weight 7500 smaller than reported previously.
- Subjects :
- Amino Acids analysis
Animals
Chromatography, Gel
Cross-Linking Reagents chemistry
Dimerization
Dimethyl Suberimidate chemistry
Humans
L-Serine Dehydratase isolation & purification
L-Serine Dehydratase metabolism
Lasers
Male
Molecular Weight
Protein Structure, Quaternary
Rats
Rats, Sprague-Dawley
Sheep
L-Serine Dehydratase chemistry
Liver enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1357-2725
- Volume :
- 34
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The international journal of biochemistry & cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 11906824
- Full Text :
- https://doi.org/10.1016/s1357-2725(01)00146-7