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AMPA-induced Ca(2+) influx in cultured rat cortical nonpyramidal neurones: pharmacological characterization using fura-2 microfluorimetry.
- Source :
-
European journal of pharmacology [Eur J Pharmacol] 2002 Mar 01; Vol. 438 (1-2), pp. 53-62. - Publication Year :
- 2002
-
Abstract
- Immunocytochemical and Co(2+) uptake studies revealed that in primary cultures of rat cortical neurones, the majority of neurones are gamma-aminobutyric acid (GABA) immunopositive and can express Ca(2+)-permeable alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) receptors. By fura-2 microfluorimetry, it was shown that the stimulation with the selective agonist (S)-AMPA (0.3-300 microM) induced a concentration-dependent but cell-variable increase in intracellular Ca(2+) concentration ([Ca(2+)](i)) (EC(50) value 7.4 microM) in more than 80% of the medium-sized multipolar neurones studied. The AMPA-induced rise in [Ca(2+)](i) seems to be due to Ca(2+) entry through AMPA receptor channels, because the response was abolished in Ca(2+)-free solution and by AMPA receptor selective antagonists, but was not significantly influenced by cyclopiazonic acid, an inhibitor of the endoplasmatic Ca(2+)-ATPase, by selective N-methyl-D-aspartic acid (NMDA) receptor antagonists, as well as the Na(+) channel blocker tetrodotoxin and the majority of tested Ca(2+) channel blockers. In conclusion, the results indicate that the cerebral cortical neurones in culture represent mostly GABAergic interneurone-like cells and the majority of them possess Ca(2+)-permeable AMPA receptors, important for intracellular signal transduction and neuronal plasticity.
- Subjects :
- 6-Cyano-7-nitroquinoxaline-2,3-dione pharmacology
Animals
Benzodiazepines pharmacology
Benzothiadiazines pharmacology
Cells, Cultured
Cerebral Cortex cytology
Cerebral Cortex metabolism
Cobalt pharmacokinetics
Diazoxide pharmacology
Dose-Response Relationship, Drug
Excitatory Amino Acid Antagonists pharmacology
Fluorescence
Fluorometry methods
Fura-2
Immunohistochemistry
N-Methylaspartate pharmacology
Neurons metabolism
Piperazines pharmacology
Quinoxalines pharmacology
Rats
Receptors, AMPA antagonists & inhibitors
Receptors, N-Methyl-D-Aspartate antagonists & inhibitors
gamma-Aminobutyric Acid analysis
Calcium metabolism
Cerebral Cortex drug effects
Neurons drug effects
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2999
- Volume :
- 438
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- European journal of pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 11906710
- Full Text :
- https://doi.org/10.1016/s0014-2999(02)01296-7