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[Identification of new substrates of cyclin-activated kinases in breast cancers].
- Source :
-
Verhandlungen der Deutschen Gesellschaft fur Pathologie [Verh Dtsch Ges Pathol] 2001; Vol. 85, pp. 269-74. - Publication Year :
- 2001
-
Abstract
- Mitogenic and growth-inhibitory signals influence cell-cycle progression through their action on a family of cyclin-dependent kinases (cdks). The activity of cdk complexes is regulated in part by the association of a cyclin partner that acts as a positive effector. These cyclin/CDK complexes promote cell cycle progression by the phosphorylation of key substrates. Cyclin D1 and E are frequently overexpressed in breast cancers and cyclin E overexpression has been correlated with a poor prognostic outcome. The in vivo substrates of cyclin E/CDK2 are, however, not well defined. We screened for cyclin E/CDK2 substrates in nuclear extracts of breast cancer cells as well as using a spotted-array protein library with purified active cyclin E/CDK2 complexes that were expressed in and purified from insect cells. Using this technique several potential cyclin E/CDK2 substrates were isolated. Further work is presently underway to identify these substrates and verify their authenticity as in vivo substrates of cyclin E/CDK2. These potential new substrates will help to unravel the highly complex mechanisms of cell cycle control and perhaps offer new targets for the diagnosis and/or treatment of breast cancer patients.
Details
- Language :
- German
- ISSN :
- 0070-4113
- Volume :
- 85
- Database :
- MEDLINE
- Journal :
- Verhandlungen der Deutschen Gesellschaft fur Pathologie
- Publication Type :
- Academic Journal
- Accession number :
- 11894409