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Angiotensin II AT(2) receptor inhibits smooth muscle cell migration via fibronectin cell production and binding.
- Source :
-
American journal of physiology. Cell physiology [Am J Physiol Cell Physiol] 2002 Apr; Vol. 282 (4), pp. C654-64. - Publication Year :
- 2002
-
Abstract
- To explore the vascular function of the angiotensin II (ANG II) AT(2) receptor subtype (AT(2)R), we generated a vascular smooth muscle cell (SMC) line expressing the AT(2)R (SMC-vAT(2)). The involvement of AT(2)R in the motility response of SMCs was examined in SMC-vAT(2) cells and their controls (SMC-v) cultured on either laminin or fibronectin matrix proteins with the agarose drop technique. All experiments were conducted in the presence of a saturating concentration of losartan to inactivate the AT(1)R subtype. Under basal conditions, both cell lines migrated outside drops, but on laminin only. Treatment with ANG II significantly inhibited the migration of SMC-vAT(2) but not SMC-v cells, and this effect was prevented by the AT(2)R antagonist CGP-42112A. The decreased migration of SMC-vAT(2) was not associated with changes in cell growth, cytoskeleton stiffness, or smooth muscle actin, desmin, and tenascin expression. However, it was correlated with increased synthesis and binding of fibronectin. Both responses were prevented by incubation with selective AT(2)R antagonists. Addition of GRGDTP peptide, which prevents cell attachment of fibronectin, reversed the AT(2)R inhibitory effect on SMC-vAT(2) migration. These results suggest that activated ANG II AT(2)R inhibits SMC migration via cellular fibronectin synthesis and associated cell binding.
- Subjects :
- Animals
Cell Adhesion physiology
Cells, Cultured
Cytoskeleton metabolism
Extracellular Matrix Proteins metabolism
Gene Expression
Gene Transfer Techniques
Humans
Iodine Radioisotopes
Laminin metabolism
Laminin pharmacology
Rats
Receptor, Angiotensin, Type 2
Receptors, Angiotensin genetics
Retroviridae genetics
Cell Movement physiology
Fibronectins metabolism
Muscle, Smooth, Vascular cytology
Muscle, Smooth, Vascular metabolism
Receptors, Angiotensin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0363-6143
- Volume :
- 282
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Cell physiology
- Publication Type :
- Academic Journal
- Accession number :
- 11880254
- Full Text :
- https://doi.org/10.1152/ajpcell.00318.2001