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Angiotensin II AT(2) receptor inhibits smooth muscle cell migration via fibronectin cell production and binding.

Authors :
Chassagne C
Adamy C
Ratajczak P
Gingras B
Teiger E
Planus E
Oliviero P
Rappaport L
Samuel JL
Meloche S
Source :
American journal of physiology. Cell physiology [Am J Physiol Cell Physiol] 2002 Apr; Vol. 282 (4), pp. C654-64.
Publication Year :
2002

Abstract

To explore the vascular function of the angiotensin II (ANG II) AT(2) receptor subtype (AT(2)R), we generated a vascular smooth muscle cell (SMC) line expressing the AT(2)R (SMC-vAT(2)). The involvement of AT(2)R in the motility response of SMCs was examined in SMC-vAT(2) cells and their controls (SMC-v) cultured on either laminin or fibronectin matrix proteins with the agarose drop technique. All experiments were conducted in the presence of a saturating concentration of losartan to inactivate the AT(1)R subtype. Under basal conditions, both cell lines migrated outside drops, but on laminin only. Treatment with ANG II significantly inhibited the migration of SMC-vAT(2) but not SMC-v cells, and this effect was prevented by the AT(2)R antagonist CGP-42112A. The decreased migration of SMC-vAT(2) was not associated with changes in cell growth, cytoskeleton stiffness, or smooth muscle actin, desmin, and tenascin expression. However, it was correlated with increased synthesis and binding of fibronectin. Both responses were prevented by incubation with selective AT(2)R antagonists. Addition of GRGDTP peptide, which prevents cell attachment of fibronectin, reversed the AT(2)R inhibitory effect on SMC-vAT(2) migration. These results suggest that activated ANG II AT(2)R inhibits SMC migration via cellular fibronectin synthesis and associated cell binding.

Details

Language :
English
ISSN :
0363-6143
Volume :
282
Issue :
4
Database :
MEDLINE
Journal :
American journal of physiology. Cell physiology
Publication Type :
Academic Journal
Accession number :
11880254
Full Text :
https://doi.org/10.1152/ajpcell.00318.2001