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Human myosin-Vc is a novel class V myosin expressed in epithelial cells.
- Source :
-
Journal of cell science [J Cell Sci] 2002 Mar 01; Vol. 115 (Pt 5), pp. 991-1004. - Publication Year :
- 2002
-
Abstract
- Class V myosins are one of the most ancient and widely distributed groups of the myosin superfamily and are hypothesized to function as motors for actin-dependent organelle transport. We report the discovery and initial characterization of a novel member of this family, human myosin-Vc (Myo5c). The Myo5c protein sequence shares approximately 50% overall identity with the two other class V myosins in vertebrates, myosin-Va (Myo5a) and myosin-Vb (Myo5b). Systematic analysis of the mRNA and protein distribution of these myosins indicates that Myo5a is most abundant in brain, whereas Myo5b and Myo5c are expressed chiefly in non-neuronal tissues. Myo5c is particularly abundant in epithelial and glandular tissues including pancreas, prostate, mammary, stomach, colon and lung. Immunolocalization in colon and exocrine pancreas indicates that Myo5c is expressed chiefly in epithelial cells. A dominant negative approach using a GFP-Myo5c tail construct in HeLa cells reveals that the Myo5c tail selectively colocalizes with and perturbs a membrane compartment containing the transferrin receptor and rab8. Transferrin also accumulates in this compartment, suggesting that Myo5c is involved in transferrin trafficking. As a class V myosin of epithelial cells, Myo5c is likely to power actin-based membrane trafficking in many physiologically crucial tissues of the human body.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Cell Compartmentation genetics
Cloning, Molecular
Colon cytology
Colon metabolism
Green Fluorescent Proteins
HeLa Cells
Humans
Luminescent Proteins genetics
Mice
Molecular Sequence Data
Myosins genetics
Pancreas cytology
Pancreas metabolism
Protein Structure, Tertiary physiology
Receptors, Transferrin metabolism
Transferrin metabolism
Viscera cytology
Viscera metabolism
rab GTP-Binding Proteins metabolism
Epithelial Cells metabolism
Intracellular Membranes metabolism
Myosin Type V genetics
Myosin Type V metabolism
Myosins isolation & purification
Protein Transport genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9533
- Volume :
- 115
- Issue :
- Pt 5
- Database :
- MEDLINE
- Journal :
- Journal of cell science
- Publication Type :
- Academic Journal
- Accession number :
- 11870218
- Full Text :
- https://doi.org/10.1242/jcs.115.5.991