Back to Search
Start Over
Caveolin-1 expression in ovarian carcinoma is MDR1 independent.
- Source :
-
American journal of clinical pathology [Am J Clin Pathol] 2002 Feb; Vol. 117 (2), pp. 225-34. - Publication Year :
- 2002
-
Abstract
- We studied the role of caveolin-1 in tumor progression and prognosis in serous ovarian carcinoma and the association between caveolin-1 and MDR1 expression. The study involved immunohistochemical analysis for caveolin-1 and P-glycoprotein (P-gp) expression in 75 effusions and 90 solid lesions from ovarian and primary peritoneal carcinoma; in situ hybridization for MDR1 messenger RNA (mRNA) expression in 62 effusions and all 90 tumors; and reverse transcription-polymerase chain reaction (RT-PCR) for caveolin-1 mRNA expression in 23 effusions. Immunohistochemical analysis localized caveolin-1 to the cell membrane in 43 effusions and 24 tumors. P-gp membrane expression was detected in 14 effusions and 11 tumors; MDR1 mRNA, in 20 effusions and 30 tumors. Caveolin-1 mRNA was expressed in 19 effusions. Caveolin-1 protein expression showed no association with that of P-gp protein or MDR1 mRNA. The expression of all markers was similar in carcinoma cells in pleural and peritoneal effusions. Caveolin-1 is a novel diagnostic marker for effusions; expression is moderately elevated in tumor cells in effusions, possibly owing to altered signal transduction and metabolism in cancer cells at this site. Expression seems MDR1 independent.
- Subjects :
- ATP Binding Cassette Transporter, Subfamily B, Member 1 genetics
Adult
Aged
Ascitic Fluid pathology
Biomarkers, Tumor biosynthesis
Biomarkers, Tumor genetics
Carcinoma pathology
Caveolin 1
Caveolins genetics
Female
Humans
Immunohistochemistry
In Situ Hybridization
Middle Aged
Neoplasm, Residual diagnosis
Ovarian Neoplasms pathology
Peritoneal Neoplasms metabolism
Peritoneal Neoplasms pathology
Pleural Effusion, Malignant pathology
Pleural Neoplasms secondary
RNA, Messenger analysis
RNA, Messenger biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
ATP Binding Cassette Transporter, Subfamily B, Member 1 biosynthesis
Carcinoma diagnosis
Carcinoma metabolism
Caveolins biosynthesis
Ovarian Neoplasms diagnosis
Ovarian Neoplasms metabolism
Pleural Neoplasms metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9173
- Volume :
- 117
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- American journal of clinical pathology
- Publication Type :
- Academic Journal
- Accession number :
- 11863219
- Full Text :
- https://doi.org/10.1309/u40r-1bn4-6kj3-bdg3