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RANK (receptor activator of nuclear factor kappa B) and RANK ligand are expressed in giant cell tumors of bone.
- Source :
-
American journal of clinical pathology [Am J Clin Pathol] 2002 Feb; Vol. 117 (2), pp. 210-6. - Publication Year :
- 2002
-
Abstract
- In giant cell tumors of bone (GCTBs), the mesenchymal stromal cells are the neoplastic cells and induce recruitment and formation of osteoclasts (OCs). Studies on recently discovered members of the tumor necrosis factor receptor-ligand family have demonstrated a crucial role of RANKL (receptor activator of nuclear factor kappa B [RANK] ligand) expressed by osteoblast/stromal cells and of its receptor RANK expressed by OCs during OC differentiation and activation. OCs typically are present in large numbers in GCTBs, suggesting that these tumors may contain cells expressing factors that stimulate OC precursor recruitment and differentiation. We used immunohistochemical analysis to study RANKL and RANK expression in 5 GCTBs. Multinucleated cells and some mononuclear cells showed strong positive staining with anti-RANK antibodies; RANKL was present in a subset of mononuclear cells that did not express the hematopoietic lineage cell marker CD45, a feature that identified them as mesenchymal tumor cells. Our results suggest that RANKL expression may have a role in the pathogenesis of GCTBs and in the formation of the large OC population present in these tumors.
- Subjects :
- Animals
Antibody Specificity
COS Cells
Carrier Proteins analysis
Carrier Proteins genetics
Cell Count
DNA, Complementary metabolism
Gene Expression
Glycoproteins analysis
Glycoproteins genetics
Humans
Immunohistochemistry
Leukocyte Common Antigens analysis
Leukocyte Common Antigens biosynthesis
Leukocytes, Mononuclear metabolism
Leukocytes, Mononuclear pathology
Membrane Glycoproteins analysis
Membrane Glycoproteins genetics
Mesoderm metabolism
Mesoderm pathology
Osteoblasts metabolism
Osteoblasts pathology
Osteoclasts metabolism
Osteoclasts pathology
Osteoprotegerin
RANK Ligand
Receptor Activator of Nuclear Factor-kappa B
Receptors, Cytoplasmic and Nuclear analysis
Receptors, Cytoplasmic and Nuclear genetics
Receptors, Tumor Necrosis Factor
Transfection
Carrier Proteins biosynthesis
Giant Cell Tumor of Bone metabolism
Giant Cell Tumor of Bone pathology
Glycoproteins biosynthesis
Membrane Glycoproteins biosynthesis
Receptors, Cytoplasmic and Nuclear biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9173
- Volume :
- 117
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- American journal of clinical pathology
- Publication Type :
- Academic Journal
- Accession number :
- 11863217
- Full Text :
- https://doi.org/10.1309/BPET-F2PE-P2BD-J3P3