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Identification of BING-4 cancer antigen translated from an alternative open reading frame of a gene in the extended MHC class II region using lymphocytes from a patient with a durable complete regression following immunotherapy.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2002 Mar 01; Vol. 168 (5), pp. 2402-7. - Publication Year :
- 2002
-
Abstract
- Multiple human cancer Ags have been identified, although little is known concerning which would be most effectively used in cancer immunotherapy. To gain insight into the selection of appropriate Ags, the immunologic reactivity of a patient who had a durable complete regression of melanoma metastases was measured. PBMCs were directly cloned using the monoclonal anti-CD3 Ab OKT3 and IL-2 without any bias introduced by previous culture. A lymphocyte clone recognized a previously unknown shared melanoma Ag that was identified as the BING-4 protein encoded in a gene-rich region of the extended class II MHC. The HLA-A2-restricted BING-4 immunodominant peptide was translated from a 10-aa-long alternative open reading frame. In vitro sensitization against this peptide generated lymphocytes reactive against HLA-A2(+) melanomas. Real-time semiquantitative RT-PCR analysis revealed that 8 of 15 melanoma cell lines overexpressed BING-4, and this correlated with recognition by lymphocytes. Overexpression was not found in normal tissues or other tumor types. Thus, BING-4 represents another candidate Ag for possible use in the immunotherapy of patients with melanoma.
- Subjects :
- Amino Acid Sequence
Antigens, Neoplasm biosynthesis
Cell Line
Clone Cells
Cloning, Molecular
Humans
Interferon-gamma biosynthesis
Melanoma pathology
Melanoma therapy
Molecular Sequence Data
Neoplasm Metastasis
Open Reading Frames
Peptides chemistry
Peptides immunology
Protein Biosynthesis
RNA, Messenger biosynthesis
Tumor Cells, Cultured
Antigens, Neoplasm genetics
Antigens, Neoplasm immunology
Genes, MHC Class II
Melanoma immunology
T-Lymphocytes, Cytotoxic immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 168
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 11859131
- Full Text :
- https://doi.org/10.4049/jimmunol.168.5.2402