Back to Search Start Over

[Testing and isolation of high-purity restriction endonucleases].

[Testing and isolation of high-purity restriction endonucleases].

Authors :
Puchkova LI
Ushakova TA
Mikhaĭlova VK
Serov GD
Krivopalova GN
Repin VE
Source :
Prikladnaia biokhimiia i mikrobiologiia [Prikl Biokhim Mikrobiol] 2002 Jan-Feb; Vol. 38 (1), pp. 20-4.
Publication Year :
2002

Abstract

A new method of testing restriction nucleases is proposed. This method is based on high-temperature treatment of crude cell extracts. Disrupted cells were heated at 50-60 degrees C, centrifuged, and assayed for restrictases. This method provides the opportunity for screening new enzymes in microbial strains enriched with nonspecific restrictases. High-temperature treatment of cell extracts of certain producers reduces the number of steps of the procedure used for isolating high-purity restrictases; the resulting preparations are capable of maintaining high enzymatic activity during long-term storage. It was shown that high-temperature treatment can be applied not only to thermophilic but also to mesophilic strains of microorganisms of different taxa.

Details

Language :
Russian
ISSN :
0555-1099
Volume :
38
Issue :
1
Database :
MEDLINE
Journal :
Prikladnaia biokhimiia i mikrobiologiia
Publication Type :
Academic Journal
Accession number :
11852561