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[Testing and isolation of high-purity restriction endonucleases].
[Testing and isolation of high-purity restriction endonucleases].
- Source :
-
Prikladnaia biokhimiia i mikrobiologiia [Prikl Biokhim Mikrobiol] 2002 Jan-Feb; Vol. 38 (1), pp. 20-4. - Publication Year :
- 2002
-
Abstract
- A new method of testing restriction nucleases is proposed. This method is based on high-temperature treatment of crude cell extracts. Disrupted cells were heated at 50-60 degrees C, centrifuged, and assayed for restrictases. This method provides the opportunity for screening new enzymes in microbial strains enriched with nonspecific restrictases. High-temperature treatment of cell extracts of certain producers reduces the number of steps of the procedure used for isolating high-purity restrictases; the resulting preparations are capable of maintaining high enzymatic activity during long-term storage. It was shown that high-temperature treatment can be applied not only to thermophilic but also to mesophilic strains of microorganisms of different taxa.
Details
- Language :
- Russian
- ISSN :
- 0555-1099
- Volume :
- 38
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Prikladnaia biokhimiia i mikrobiologiia
- Publication Type :
- Academic Journal
- Accession number :
- 11852561