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Epitope tagging genomic DNA using a CD-tagging Tn10 minitransposon.

Authors :
Telmer CA
Berget PB
Ballou B
Murphy RF
Jarvik JW
Source :
BioTechniques [Biotechniques] 2002 Feb; Vol. 32 (2), pp. 422-4, 426, 428-30.
Publication Year :
2002

Abstract

Here, we describe an efficient system for epitope tagging cloned genes by CD tagging using a mini-Tn10 transposon delivery vector. The system was tested against a lambdaFIX genomic clone of the human nucleolin gene. Transfection of HeLa cells with the tagged gene led to the expression of both the appropriately spliced tagged transcript and the appropriately localized tagged protein.

Subjects

Subjects :
Bacteriophage lambda genetics
DNA, Recombinant chemistry
Escherichia coli genetics
Green Fluorescent Proteins
HeLa Cells
Humans
Introns
Luminescent Proteins genetics
Reverse Transcriptase Polymerase Chain Reaction
Transfection
Transposases genetics
Nucleolin
Cloning, Molecular methods
DNA Transposable Elements
DNA, Recombinant analysis
Phosphoproteins genetics
RNA-Binding Proteins genetics

Details

Language :
English
ISSN :
0736-6205
Volume :
32
Issue :
2
Database :
MEDLINE
Journal :
BioTechniques
Publication Type :
Academic Journal
Accession number :
11848418
Full Text :
https://doi.org/10.2144/02322rr04
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