Phage antibodies from combinatorial library neutralize vaccinia virus.

Authors :: Tikunova NV
Morozova VV
Batanova TA
Belanov EF
Bormotov NI
Ilyichev AA
Source :: Human antibodies [Hum Antibodies] 2001; Vol. 10 (3-4), pp. 95-9.
Publication Year :: 2001
Abstract: The library of human scFv antibodies displayed on the surface of bacteriophages was panned against Vaccinia virus (VACV), strain Elstree. 75% binding with Vaccinia virus. 5 clones were characterized for their binding with VACV and their ability to neutralize VACV in plaque reduction neutralization test (PRNT). Antibodies from the clones were obtained as soluble individual molecules and their binding activities were confirmed in ELISA.