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Docking protein SNT1 is a critical mediator of fibroblast growth factor signaling during Xenopus embryonic development.
- Source :
-
Developmental dynamics : an official publication of the American Association of Anatomists [Dev Dyn] 2002 Mar; Vol. 223 (2), pp. 216-28. - Publication Year :
- 2002
-
Abstract
- The docking protein SNT1/FRS2 (fibroblast growth factor receptor substrate 2) is implicated in the transmission of extracellular signals from several growth factor receptors to the mitogen-activated protein (MAP) kinase signaling cascade, but its biological function during development is not well characterized. Here, we show that the Xenopus homolog of mammalian SNT1/FRS-2 (XSNT1) plays a critical role in the appropriate formation of mesoderm-derived tissue during embryogenesis. XSNT1 has an expression pattern that is quite similar to the fibroblast growth factor receptor-1 (FGFR1) during Xenopus development. Ectopic expression of XSNT1 markedly enhanced the embryonic defects induced by an activated FGF receptor, and increased the MAP kinase activity as well as the expression of a mesodermal marker in response to FGF receptor signaling. A loss-of-function study using antisense XSNT1 morpholino oligonucleotides (XSNT-AS) shows severe malformation of trunk and posterior structures. Moreover, XSNT-AS disrupts muscle and notochord formation, and inhibits FGFR-induced MAP kinase activation. In ectodermal explants, XSNT-AS blocks FGFR-mediated induction of mesoderm and the accompanying elongation movements. Our results indicate that XSNT1 is a critical mediator of FGF signaling and is required for early Xenopus development.<br /> (Copyright 2002 Wiley-Liss, Inc.)
- Subjects :
- Adaptor Proteins, Signal Transducing
Amino Acid Sequence
Animals
Embryo, Nonmammalian metabolism
Embryonic Development
Gene Expression Regulation, Developmental
In Situ Hybridization
Membrane Proteins biosynthesis
Membrane Proteins genetics
Mesoderm metabolism
Molecular Sequence Data
Morphogenesis
Oligodeoxyribonucleotides, Antisense pharmacology
Phosphoproteins biosynthesis
Phosphoproteins genetics
Receptor Protein-Tyrosine Kinases biosynthesis
Receptor Protein-Tyrosine Kinases genetics
Receptor, Fibroblast Growth Factor, Type 1
Receptors, Fibroblast Growth Factor biosynthesis
Receptors, Fibroblast Growth Factor genetics
Sequence Alignment
Sequence Homology, Amino Acid
Species Specificity
Vertebrates genetics
Xenopus Proteins biosynthesis
Xenopus Proteins genetics
Xenopus laevis genetics
Xenopus laevis metabolism
Fibroblast Growth Factor 1 physiology
MAP Kinase Signaling System
Membrane Proteins physiology
Phosphoproteins physiology
Receptor Protein-Tyrosine Kinases physiology
Receptors, Fibroblast Growth Factor physiology
Xenopus Proteins physiology
Xenopus laevis embryology
Subjects
Details
- Language :
- English
- ISSN :
- 1058-8388
- Volume :
- 223
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Developmental dynamics : an official publication of the American Association of Anatomists
- Publication Type :
- Academic Journal
- Accession number :
- 11836786
- Full Text :
- https://doi.org/10.1002/dvdy.10048