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Suppression of tumor growth and pulmonary metastasis in murine osteosarcoma using gene therapy.
- Source :
-
Oncology reports [Oncol Rep] 2002 Mar-Apr; Vol. 9 (2), pp. 337-40. - Publication Year :
- 2002
-
Abstract
- We evaluated the effect of gene therapy in the murine osteosarcoma cell line, LM8, which preferentially metastasizes to the lungs. LM8 cells were transduced with the gene for a herpes simplex virus thymidine kinase (HSV-tk) or Escherichia coli beta-galactosidase (lacZ). We investigated the cytotoxicity of LM8 cells bearing an HSV-tk gene after treatment with ganciclovir (GCV). LM8 cells bearing an HSV-tk gene were more sensitive than non-transduced cells. The remarkable inhibition of tumor growth and pulmonary metastases was confirmed in vivo. Our findings indicated that GCV kills tumor cells transduced with HSV-tk in vitro and in vivo.
- Subjects :
- Adenoviridae genetics
Animals
Bone Neoplasms genetics
Bone Neoplasms pathology
Ganciclovir therapeutic use
Humans
Lung Neoplasms genetics
Lung Neoplasms secondary
Male
Mice
Mice, Inbred CBA
Neoplasm Transplantation
Osteosarcoma genetics
Osteosarcoma secondary
Simplexvirus enzymology
Simplexvirus genetics
Survival Rate
Thymidine Kinase genetics
Transduction, Genetic
Transfection
Tumor Cells, Cultured
Bone Neoplasms therapy
Genetic Therapy methods
Lung Neoplasms therapy
Osteosarcoma therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1021-335X
- Volume :
- 9
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Oncology reports
- Publication Type :
- Academic Journal
- Accession number :
- 11836603