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Analysis of NaCl transport in thin ascending limb of Henle's loop in CLC-K1 null mice.
- Source :
-
American journal of physiology. Renal physiology [Am J Physiol Renal Physiol] 2002 Mar; Vol. 282 (3), pp. F451-7. - Publication Year :
- 2002
-
Abstract
- To characterize the nature of NaCl transport in the thin ascending limb (tAL), we examined the transport properties of Na(+) and Cl(-) using in vitro microperfusion of the tAL in CLC-K1 null mice. In the presence of a transmural NaCl concentration gradient (100 mM higher in the lumen), the transepithelial diffusion voltage (V(d)) was 15.5 +/- 1.0 and -7.6 +/- 1.4 mV in CLC-K1(+/+) and CLC-K1(-/-) mice, respectively. Neither Cl(-) transport inhibitor 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB) nor acidification of the bathing fluid changed the V(d) values in CLC-K1(-/-) mice. The addition of 300 microg/ml protamine, a selective blocker of paracellular conductance, to the bath increased the V(d) values by 5.6 +/- 0.7 and 12.6 +/- 1.5 mV (P < 0.001) in CLC-K1(+/+) and CLC-K1(-/-) mice, respectively. Although efflux coefficients of (36)Cl were significantly decreased in CLC-K1(-/-) mice (188.3 +/- 25.6 in 4 tubules vs. 17.2 +/- 7.0 x 10(-5) cm/s in 6 tubules), those of (22)Na were not different between CLC-K1(+/+) and CLC-K1(-/-) mice. These results clearly indicate that the major component of Cl(-) transport sensitive to NPPB or pH is mediated by CLC-K1 in the tAL.
- Subjects :
- Angiogenesis Inhibitors pharmacology
Animals
Anticoagulants pharmacology
Biological Transport drug effects
Biological Transport physiology
Chloride Channels metabolism
Electric Conductivity
Heparin pharmacology
Hydrogen-Ion Concentration
Kidney Medulla metabolism
Mice
Mice, Knockout
Nitrobenzoates pharmacology
Protamines pharmacology
Radioisotopes
Sodium Channel Blockers pharmacology
Sodium Radioisotopes
Chloride Channels genetics
Kidney Concentrating Ability physiology
Loop of Henle metabolism
Sodium Chloride metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1931-857X
- Volume :
- 282
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Renal physiology
- Publication Type :
- Academic Journal
- Accession number :
- 11832425
- Full Text :
- https://doi.org/10.1152/ajprenal.0192.2001