Determination of some anti-human immunodeficiency virus nucleosides by capillary zone electrophoresis-tandem mass spectrometry.

Authors :: Cahours X
Morin P
Dessans H
Agrofoglio LA
Source :: Electrophoresis [Electrophoresis] 2002 Jan; Vol. 23 (1), pp. 88-92.
Publication Year :: 2002
Abstract: We describe the analysis of two potent anti-human immunodeficiency virus reverse transcriptase (RT-HIV) inhibitors, zidovudine (AZT) and stavudine (d4T), among a pool of natural nucleosides (A, C, G, U, T) by capillary zone electrophoresis (CZE)-ionspray-tandem mass spectrometry (MS/MS) in positive ion mode. Several volatile formic acid-ammonia buffers having the same ionic strength (50 mM) but different pH values varying in the 9-11 pH range were prepared and tested to determine the best electrophoretic migration conditions. Quantitative CZE-MS/MS analysis was performed using selected reaction monitoring (SRM) mode. Finally, this CZE-MS/MS procedure opens the possibility for future determination of several nucleoside RT-HIV inhibitors in cell pool samples.

Subjects :: Electrophoresis, Capillary methods
Hydrogen-Ion Concentration
Osmolar Concentration
Reference Standards
Reverse Transcriptase Inhibitors analysis
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization standards
Stavudine analysis
Zidovudine analysis
Anti-HIV Agents analysis
Electrophoresis, Capillary standards
Nucleosides analysis
Spectrometry, Mass, Electrospray Ionization methods

Language :: English
ISSN :: 0173-0835
Volume :: 23
Issue :: 1
Database :: MEDLINE
Journal :: Electrophoresis
Publication Type :: Academic Journal
Accession number :: 11824626
Full Text :: https://doi.org/10.1002/1522-2683(200201)23:1<88::AID-ELPS88>3.0.CO;2-Q

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