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Cloning and expression of NS3 cDNA fragment of HCV genome of Hebei isolate in E.coli.
- Source :
-
World journal of gastroenterology [World J Gastroenterol] 1998 Apr; Vol. 4 (2), pp. 165-168. - Publication Year :
- 1998
-
Abstract
- AIM:To obtain greater antigenicity of HCV NS3 protein.METHODS:The HCV NS3 cDNA fragment was amplified by reverse transcription polymerase chain reaction from the sera of the HCV infected patients.The DNA sequence was determined by dideoxy-mediated chain termination method using T7 polymerase.HCV NS3 protein was expressed in E. coli.RESULTS:Sequence analysis indicated that the HCV isolate of this study belongs to HCV-II; SDS-PAGE demonstrated an M(r) 23800 and an M(r) 22000 recombinant protein band which amount to 14% and 11% of the total bacterial proteins separately.Western blotting and ELISA showed NS3 protein possessed greater antigenicity.CONCLUSION:Recombinant HCV NS3 protein was expressed successfully, which provided the basis for developing HCV diagnostic reagents.
Details
- Language :
- English
- ISSN :
- 2219-2840
- Volume :
- 4
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- World journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 11819265
- Full Text :
- https://doi.org/10.3748/wjg.v4.i2.165