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A novel bifunctional molybdo-enzyme catalyzing both decarboxylation of indolepyruvate and oxidation of indoleacetaldehyde from a thermoacidophilic archaeon, Sulfolobus sp. strain 7.
- Source :
-
FEBS letters [FEBS Lett] 2002 Jan 16; Vol. 510 (3), pp. 196-200. - Publication Year :
- 2002
-
Abstract
- An enzyme, which catalyzes both decarboxylation of indolepyruvate and subsequent oxidation of indoleacetaldehyde into indoleacetate, was purified from a thermoacidophilic archaeon, Sulfolobus sp. strain 7. The enzyme showed a M(r) of 280 kDa on gel filtration and was composed of three subunits (a, 89; b, 30; and c, 19 kDa), possibly in a stoichiometry of 2:2:2. Mo and Fe were detected. Thiamine pyrophosphate was absent. Biotin was suggested to bind to the b-subunit. The first step, the decarboxylation reaction, was specific for 2-oxoacids with an aromatic group, while in the second reaction, various aldehydes including glyceraldehyde, which is a glycolytic intermediate in the organism, were oxidized.
- Subjects :
- Aldehyde Oxidase
Aldehyde Oxidoreductases chemistry
Aldehyde Oxidoreductases isolation & purification
Amino Acid Sequence
Archaeal Proteins chemistry
Archaeal Proteins isolation & purification
Biotin chemistry
Carboxy-Lyases isolation & purification
Chromatography, Gel
Chromatography, High Pressure Liquid
Enzyme Stability
Hydrogen-Ion Concentration
Indoles chemistry
Molecular Sequence Data
Molecular Weight
Multienzyme Complexes isolation & purification
Oxidation-Reduction
Oxidoreductases isolation & purification
Protein Subunits
Sequence Analysis, Protein
Sequence Homology, Amino Acid
Substrate Specificity
Temperature
Carboxy-Lyases chemistry
Molybdenum chemistry
Multienzyme Complexes chemistry
Oxidoreductases chemistry
Sulfolobus enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 510
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 11801253
- Full Text :
- https://doi.org/10.1016/s0014-5793(01)03259-8