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Lymphocyte adhesion to Candida albicans.
- Source :
-
Infection and immunity [Infect Immun] 2002 Feb; Vol. 70 (2), pp. 517-27. - Publication Year :
- 2002
-
Abstract
- Adherence of lymphocytes to the fungus is the first step in the direct lymphocyte-mediated antifungal effect against Candida albicans. In this study we identified macrophage-1 antigen (Mac-1) (CD11b/CD18, alpha(M)/beta(2)) as the lymphocyte surface structure responsible for the adhesion of activated lymphocytes to the hyphal form of the fungus. Antibodies specific for epitopes of the alpha-subunit (CD11b) and the beta(2)-subunit (CD18) of Mac-1 were shown to completely eliminate lymphocyte adhesion to C. albicans hyphae. Lymphocyte adhesion to C. albicans was also inhibited significantly by known ligands of Mac-1, including the extracellular matrix proteins laminin and fibrinogen, as well as engineered peptides containing arginine-glycine-aspartic acid sequences and the disintegrin echistatin. N-Acetyl-D-glucosamine and beta-glucan, which inhibit Mac-1-mediated adhesion to the yeast, blocked lymphocyte adhesion to hyphae. NIH 3T3 fibroblast transfectants expressing human CD11b/CD18 bound to C. albicans, and their binding was inhibited by antibodies specific for CD11b/CD18. Finally, antibodies specific for CD11b/CD18 effectively inhibited the capacity of activated lymphocytes to have an antifungal effect against hyphae. Our results clearly identify Mac-1 (CD11b/CD18) as the lymphocyte surface structure that mediates activated lymphocyte adhesion to C. albicans and the resultant antifungal effect of the lymphocytes.
- Subjects :
- 3T3 Cells
Animals
Antibodies, Monoclonal immunology
CD18 Antigens genetics
Carbohydrates pharmacology
Cell Adhesion drug effects
Cell Division drug effects
Cells, Cultured
Female
Fibrinogen pharmacology
Humans
Laminin pharmacology
Leukocytes, Mononuclear cytology
Lymphocyte Activation
Lymphocytes drug effects
Macrophage-1 Antigen genetics
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Oligopeptides pharmacology
Spleen cytology
Transfection
CD18 Antigens immunology
Candida albicans immunology
Lymphocytes immunology
Macrophage-1 Antigen immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0019-9567
- Volume :
- 70
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 11796578
- Full Text :
- https://doi.org/10.1128/IAI.70.2.517-527.2002