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Neoplastic T cells in angioimmunoblastic T-cell lymphoma express CD10.
- Source :
-
Blood [Blood] 2002 Jan 15; Vol. 99 (2), pp. 627-33. - Publication Year :
- 2002
-
Abstract
- Angioimmunoblastic T-cell lymphoma (AITL) is a systemic disease involving lymph nodes, spleen, and bone marrow. Although the histologic features have been well described, the diagnosis is often challenging, as there are no specific phenotypic or molecular markers available. This study shows that the neoplastic cells of AITL can be identified by aberrant CD10 expression. Archival material from 30 cases of AITL, 10 cases of peripheral T-cell lymphoma unspecified (PTL), and 10 cases of reactive lymphoid hyperplasia were reviewed. Single and double immunostaining for CD3, CD4, CD8, CD20, CD21, CD10, BCL6, Ki67, and LMP-1 in situ hybridization for Epstein-Barr early region and polymerase chain reaction (PCR) for T-cell receptor gamma chain gene and immunoglobulin heavy chain gene were performed. Three overlapping histologic patterns with hyperplastic follicles, depleted follicles, or without follicles were identified in AITL. Of the 30 cases of AITL, 27 contained CD10(+) T cells. No CD10(+) T cells were present in the cases of PTL or reactive hyperplasia. PCR confirmed a monoclonal or oligoclonal T-cell population in 29 of 30 cases of AITL and a monoclonal B-cell population in 6 cases. Analysis of microdissected CD10(+) single cells showed that they belonged to the neoplastic clone. In conclusion CD10 is a phenotypic marker that specifically identifies the tumor cells in 90% of AITL, including the early cases. The presence of these cells distinguishes AITL from other PTLs. This finding provides an objective criterion for accurate and early diagnosis of AITL.
- Subjects :
- Adaptor Proteins, Signal Transducing
Adult
Aged
Aged, 80 and over
Antigens, CD analysis
Biopsy
Carrier Proteins analysis
Cell Separation methods
Clone Cells metabolism
Clone Cells pathology
Cytoskeletal Proteins
DNA, Viral analysis
DNA-Binding Proteins analysis
Epstein-Barr Virus Infections metabolism
Epstein-Barr Virus Infections pathology
Female
Gene Rearrangement, B-Lymphocyte, Heavy Chain
Gene Rearrangement, T-Lymphocyte
Herpesvirus 4, Human isolation & purification
Humans
Immunoblastic Lymphadenopathy metabolism
Immunoblastic Lymphadenopathy virology
Immunoenzyme Techniques
In Situ Hybridization
Intracellular Signaling Peptides and Proteins
Ki-67 Antigen analysis
LIM Domain Proteins
Lymph Nodes chemistry
Lymph Nodes pathology
Lymphoma, T-Cell metabolism
Lymphoma, T-Cell, Peripheral classification
Lymphoma, T-Cell, Peripheral metabolism
Lymphoma, T-Cell, Peripheral pathology
Male
Micromanipulation
Middle Aged
Polymerase Chain Reaction
Proto-Oncogene Proteins analysis
Proto-Oncogene Proteins c-bcl-6
Pseudolymphoma metabolism
Pseudolymphoma pathology
Transcription Factors analysis
Biomarkers, Tumor analysis
Immunoblastic Lymphadenopathy pathology
Lymphoma, T-Cell pathology
Neoplasm Proteins analysis
Neoplastic Stem Cells metabolism
Neprilysin analysis
T-Lymphocytes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0006-4971
- Volume :
- 99
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Blood
- Publication Type :
- Academic Journal
- Accession number :
- 11781247
- Full Text :
- https://doi.org/10.1182/blood.v99.2.627