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Candidate gene promoter polymorphisms and antibody response kinetics in chickens: interferon-gamma, interleukin-2, and immunoglobulin light chain.
- Source :
-
Poultry science [Poult Sci] 2001 Dec; Vol. 80 (12), pp. 1679-89. - Publication Year :
- 2001
-
Abstract
- An F2 population was produced from mating G0 highly inbred (>99%) males of two MHC-congenic Fayoumi lines with G-B1 Leghorn hens. The F2 population was essentially a full-sibship with the F1 sire line reflecting MHC effect. Adult F2 hens (n = 158) were injected twice with SRBC and whole fixed Brucella abortus (BA). Agglutinating antibody titer at 7 d after primary immunization and mean titer of the final three samples (Days 18, 32, and 63 after the second immunization) were used as parameters for primary and equilibrium phases, respectively. Secondary phase parameters of minimum (Ymin), maximum titers (Ymax) and time needed to achieve minimum (Tmin) and maximum (Tmax) titers were estimated from seven postsecondary titers with a nonlinear regression model. Three candidate genes, interferon-gamma (IFN-gamma), interleukin-2 (IL-2), and immunoglobulin G light chain (IgL) were studied. Primers for the promoter regions were designed from EMBL chicken genomic sequences. Polymorphisms between parental lines were detected by direct sequencing. Polymerase chain reaction-restriction fragment length polymorphism methods were then developed to directly detect the polymorphism. There were significant main effects (P < 0.05, general linear model analysis) of IFN-gamma polymorphism on Ymax of BA antibody and interaction of IFN-gamma by IgL on primary antibody response to SRBC and BA, and on Tmin and Ymin of antibody response to SRBC in F2 offspring of M5.1 grandsires. There were significant main effects of IFN-gamma polymorphism on Tmax of BA and interaction of IFN-gamma by IL-2 on Ymin to SRBC in F2 offspring of M15.2 grandsires. The results suggest that IFN-gamma genes play an important role in chicken primary and secondary antibody response to SRBC and BA antigens, and there exists interaction among genes for antibody production.
- Subjects :
- Agglutination Tests veterinary
Alleles
Animals
Antibody Formation genetics
Chickens classification
Female
Gene Expression Regulation genetics
Genotype
Immunoglobulin Light Chains immunology
Interferon-gamma immunology
Interleukin-2 immunology
Kinetics
Male
Polymerase Chain Reaction veterinary
Polymorphism, Restriction Fragment Length
Promoter Regions, Genetic genetics
Time Factors
Chickens genetics
Chickens immunology
Immunoglobulin Light Chains genetics
Interferon-gamma genetics
Interleukin-2 genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0032-5791
- Volume :
- 80
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Poultry science
- Publication Type :
- Academic Journal
- Accession number :
- 11771881
- Full Text :
- https://doi.org/10.1093/ps/80.12.1679