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Carbohydrate-deficient transferrin and chronic alcohol ingestion in subjects with transferrin CD-variants.
- Source :
-
Clinical chemistry and laboratory medicine [Clin Chem Lab Med] 2001 Oct; Vol. 39 (10), pp. 937-43. - Publication Year :
- 2001
-
Abstract
- Carbohydrate-deficient transferrin (CDT) is widely accepted as screening test for excessive alcohol consumption. However, results from subjects with transferrin variants must be interpreted with caution since chromatography-based methods may give false-positive results. Furthermore, due to the co-elution in HPLC or the co-migration in capillary zone electrophoresis (CZE) of the di- and trisialylated C transferrins with the tetrasialylated D peak, exact measurement of CDT is impossible in CD-variants. Therefore, in this study, we tried to offer a different solution, including only the asialo-D, asialo-C, monosialo-D, monosialo-C, disialo-D and trisialo-D transferrins in the CDT calculation and referring to a different cut-off value for CDT in transferrin CD-variants. Comparison of alcohol consumers with teetotalers demonstrated area under the receiver operating characteristic curve of 0.79 and 0.76 for carbohydrate-deficient transferrin, 0.71 and 0.71 for mean corpuscular volume and 0.51 and 0.68 for gamma-glutamyltransferase in 43 subjects with transferrin CD-variants and 225 subjects with CC-phenotypes, respectively. Since false-positive carbohydrate-deficient transferrin results due to a transferrin CD-variant have major social implications, capillary electrophoresis-based or similar methods (HPLC, FPLC) should be preferred in populations carrying a high D-allele frequency.
- Subjects :
- Adult
Aged
Alcoholism genetics
Biomarkers blood
Blood Chemical Analysis methods
Chromatography, High Pressure Liquid
Electrophoresis, Capillary methods
False Positive Reactions
Female
Humans
Male
Middle Aged
Phenotype
Temperance
Alcoholism blood
Alcoholism diagnosis
Genetic Variation
Transferrin analogs & derivatives
Transferrin analysis
Transferrin genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1434-6621
- Volume :
- 39
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Clinical chemistry and laboratory medicine
- Publication Type :
- Academic Journal
- Accession number :
- 11758606
- Full Text :
- https://doi.org/10.1515/CCLM.2001.150