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Integrin-induced epidermal growth factor (EGF) receptor activation requires c-Src and p130Cas and leads to phosphorylation of specific EGF receptor tyrosines.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2002 Mar 15; Vol. 277 (11), pp. 9405-14. Date of Electronic Publication: 2001 Dec 27. - Publication Year :
- 2002
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Abstract
- Integrin-mediated cell adhesion cooperates with growth factor receptors in the control of cell proliferation, cell survival, and cell migration. One mechanism to explain these synergistic effects is the ability of integrins to induce phosphorylation of growth factor receptors, for instance the epidermal growth factor (EGF) receptor. Here we define some aspects of the molecular mechanisms regulating integrin-dependent EGF receptor phosphorylation. We show that in the early phases of cell adhesion integrins associate with EGF receptors on the cell membrane in a macromolecular complex including the adaptor protein p130Cas and the c-Src kinase, the latter being required for adhesion-dependent assembly of the macromolecular complex. We also show that the integrin cytoplasmic tail, c-Src kinase, and the p130Cas adaptor protein are required for phosphorylation of EGF receptor in response to integrin-mediated adhesion. We show that integrins induce phosphorylation of EGF receptor on tyrosine residues 845, 1068, 1086, and 1173, but not on residue 1148, a major site of phosphorylation in response to EGF. In addition we find that integrin-mediated adhesion increases the amount of EGF receptor expressed on the cell surface. Therefore these data indicate that integrin-mediated adhesion induces assembly of a macromolecular complex containing c-Src and p130Cas and leads to phosphorylation of specific EGF receptor tyrosine residues.
- Subjects :
- CSK Tyrosine-Protein Kinase
Cell Adhesion
Cell Line
Crk-Associated Substrate Protein
ErbB Receptors analysis
ErbB Receptors chemistry
Focal Adhesion Kinase 1
Focal Adhesion Protein-Tyrosine Kinases
Humans
Integrins chemistry
Phosphorylation
Retinoblastoma-Like Protein p130
src-Family Kinases
ErbB Receptors metabolism
Integrins physiology
Phosphoproteins physiology
Protein-Tyrosine Kinases physiology
Proteins
Tyrosine metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 277
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 11756413
- Full Text :
- https://doi.org/10.1074/jbc.M109101200