Back to Search
Start Over
Orphan nuclear receptor Nurr1 is essential for Ret expression in midbrain dopamine neurons and in the brain stem.
- Source :
-
Molecular and cellular neurosciences [Mol Cell Neurosci] 2001 Dec; Vol. 18 (6), pp. 649-63. - Publication Year :
- 2001
-
Abstract
- The orphan nuclear receptor Nurr1 is essential for development of midbrain dopamine (DA) cells. In Nurr1-deficient mice, DA precursor cells fail to migrate normally, are unable to innervate target areas, and only transiently express DA cell marker genes. In the search for Nurr1-regulated genes that might explain this developmental phenotype, we found that expression of the receptor tyrosine kinase Ret is deregulated in these cells of Nurr1-deficient embryos. In addition, our analyses establish Nurr1 as an early marker for the dorsal motor nucleus (DMN) of the vagus nerve. Interestingly, Ret expression is absent also in these cells in Nurr1-targeted mice. Neuronal innervation of vagus nerve target areas appeared normal apart from a subtle disorganization of the DMN-derived nerve fibers. In conclusion, regulation of Ret by Nurr1 in midbrain DA neurons and in the DMN has implications for both embryonal development and adult physiology in which signaling by neurotrophic factors plays important roles.
- Subjects :
- Acetylcholinesterase metabolism
Aldehyde Oxidoreductases metabolism
Animals
Carrier Proteins metabolism
Female
Fetus
Gene Expression Regulation physiology
Immunohistochemistry
In Situ Hybridization
Male
Medulla Oblongata cytology
Medulla Oblongata embryology
Mesencephalon cytology
Mesencephalon embryology
Mice
Mice, Knockout
Neurons cytology
Nuclear Receptor Subfamily 4, Group A, Member 2
Proto-Oncogene Proteins c-ret
RNA, Messenger metabolism
Receptors, Cytoplasmic and Nuclear genetics
Retinal Dehydrogenase
Transcription Factors genetics
Vagus Nerve cytology
Vagus Nerve embryology
Vesicular Acetylcholine Transport Proteins
Viscera embryology
Viscera innervation
DNA-Binding Proteins
Dopamine metabolism
Drosophila Proteins
Medulla Oblongata metabolism
Membrane Transport Proteins
Mesencephalon metabolism
Neurons metabolism
Proto-Oncogene Proteins metabolism
Receptor Protein-Tyrosine Kinases metabolism
Receptors, Cytoplasmic and Nuclear deficiency
Transcription Factors deficiency
Vagus Nerve metabolism
Vesicular Transport Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 1044-7431
- Volume :
- 18
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Molecular and cellular neurosciences
- Publication Type :
- Academic Journal
- Accession number :
- 11749040
- Full Text :
- https://doi.org/10.1006/mcne.2001.1057