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Parallel extraction columns and parallel analytical columns coupled with liquid chromatography/tandem mass spectrometry for on-line simultaneous quantification of a drug candidate and its six metabolites in dog plasma.
- Source :
-
Rapid communications in mass spectrometry : RCM [Rapid Commun Mass Spectrom] 2001; Vol. 15 (22), pp. 2135-44. - Publication Year :
- 2001
-
Abstract
- A method with parallel extraction columns and parallel analytical columns (PEC-PAC) for on-line high-flow liquid chromatography/tandem mass spectrometry (LC/MS/MS) was developed and validated for simultaneous quantification of a drug candidate and its six metabolites in dog plasma. Two on-line extraction columns were used in parallel for sample extraction and two analytical columns were used in parallel for separation and analysis. The plasma samples, after addition of an internal standard solution, were directly injected onto the PEC-PAC system for purification and analysis. This method allowed the use of one of the extraction columns for analyte purification while the other was being equilibrated. Similarly, one of the analytical columns was employed to separate the analytes while the other was undergoing equilibration. Therefore, the time needed for re-conditioning both extraction and analytical columns was not added to the total analysis time, which resulted in a shorter run time and higher throughput. Moreover, the on-line column extraction LC/MS/MS method made it possible to extract and analyze all seven analytes simultaneously with good precision and accuracy despite their chemical class diversity that included primary, secondary and tertiary amines, an alcohol, an aldehyde and a carboxylic acid. The method was validated with the standard curve ranging from 5.00 to 5000 ng/mL. The intra- and inter-day precision was no more than 8% CV and the assay accuracy was between 95 and 107%.<br /> (Copyright 2001 John Wiley & Sons, Ltd.)
- Subjects :
- Alcohols analysis
Alcohols blood
Amines analysis
Amines blood
Animals
Carboxylic Acids analysis
Carboxylic Acids blood
Chromatography, Liquid standards
Dogs
Mass Spectrometry standards
Pharmaceutical Preparations blood
Quality Control
Reference Standards
Reproducibility of Results
Chromatography, Liquid instrumentation
Chromatography, Liquid methods
Mass Spectrometry instrumentation
Mass Spectrometry methods
Pharmaceutical Preparations analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0951-4198
- Volume :
- 15
- Issue :
- 22
- Database :
- MEDLINE
- Journal :
- Rapid communications in mass spectrometry : RCM
- Publication Type :
- Academic Journal
- Accession number :
- 11746878
- Full Text :
- https://doi.org/10.1002/rcm.492