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Characterization of G3BPs: tissue specific expression, chromosomal localisation and rasGAP(120) binding studies.
- Source :
-
Journal of cellular biochemistry [J Cell Biochem] 2001; Vol. 84 (1), pp. 173-87. - Publication Year :
- 2001
-
Abstract
- The G3BP (ras-GTPase-Activating Protein SH3-Domain-Binding Protein) family of proteins has been implicated in both signal transduction and RNA-metabolism. We have previously identified human G3BP-1, G3BP-2, and mouse G3BP-2. Here, we report the cloning of mouse G3BP-1, the discovery of two alternatively spliced isoforms of mouse, and human G3BP-2 (G3BP-2a and G3BP-2b), and the chromosomal localisation of human G3BP-1 and G3BP-2, which map to 5q14.2-5q33.3 and 4q12-4q24 respectively. We mapped the rasGAP(120) interactive region of the G3BP-2 isoforms and show that both G3BP-2a and G3BP-2b use an N-terminal NTF2-like domain for rasGAP(120) binding rather than several available proline-rich (PxxP) motifs found in members of the G3BPs. Furthermore, we have characterized the protein expression of both G3BP-1 and G3BP-2a/b in adult mouse tissues, and show them to be both tissue and isoform specific.<br /> (Copyright 2001 Wiley-Liss, Inc.)
- Subjects :
- Amino Acid Sequence
Animals
Chromosome Mapping
Cloning, Molecular methods
DNA Helicases
Humans
Immunohistochemistry
Insecta cytology
Insecta metabolism
Mice
Molecular Sequence Data
Poly-ADP-Ribose Binding Proteins
Protein Binding
RNA Helicases
RNA Recognition Motif Proteins
RNA-Binding Proteins metabolism
Sequence Homology
Signal Transduction physiology
Tissue Distribution physiology
Carrier Proteins metabolism
Chromosomes, Human, Pair 4
Chromosomes, Human, Pair 5
p120 GTPase Activating Protein metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0730-2312
- Volume :
- 84
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of cellular biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 11746526
- Full Text :
- https://doi.org/10.1002/jcb.1277