Back to Search
Start Over
An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine.
- Source :
-
Plant physiology [Plant Physiol] 2001 Dec; Vol. 127 (4), pp. 1556-67. - Publication Year :
- 2001
-
Abstract
- In contrast to angiosperms, pines and other gymnosperms form well-developed suspensors in somatic embryogenic cultures. This creates a useful system to study suspensor biology. In a study of gene expression during the early stages of conifer embryogenesis, we identified a transcript, PtNIP1;1, that is abundant in immature loblolly pine (Pinus taeda) zygotic and somatic embryos, but is undetectable in later-stage embryos, megagametophytes, and roots, stems, and needles from 1 year-old seedlings. Analysis of PtNIP1;1 transcript in embryo proper and suspensor tissues by reverse transcription-polymerase chain reaction suggests preferential expression in the suspensor. Based on comparisons of derived amino acid sequences, PtNIP1;1 belongs to the nodulin-like members of the major intrinsic protein superfamily branch of the aquaporin (major intrinsic protein) superfamily. Through heterologous expression in Xenopus laevis oocytes and the yeast (Saccharomyces cerevisiae) fps1(-) mutant, PtNIP1;1 has been shown to be an active aquaglyceroporin.
- Subjects :
- Amino Acid Sequence
Animals
Aquaporins metabolism
Cloning, Molecular
Eukaryotic Initiation Factor-3
Female
Fungal Proteins genetics
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Glycerol metabolism
Immediate-Early Proteins genetics
Ion Channels genetics
Membrane Proteins genetics
Molecular Sequence Data
Nuclear Proteins genetics
Oocytes
Osmotic Pressure
Pinus growth & development
Pinus taeda
Plant Proteins genetics
RNA, Messenger genetics
RNA, Messenger metabolism
Saccharomyces cerevisiae genetics
Seeds growth & development
Sequence Homology, Amino Acid
Serine Endopeptidases genetics
Xenopus laevis genetics
Aquaporins genetics
Phylogeny
Pinus genetics
Saccharomyces cerevisiae Proteins
Seeds genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0032-0889
- Volume :
- 127
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Plant physiology
- Publication Type :
- Academic Journal
- Accession number :
- 11743100