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PsaA (pneumococcal surface adhesin A) and PspA (pneumococcal surface protein A) DNA vaccines induce humoral and cellular immune responses against Streptococcus pneumoniae.
- Source :
-
Vaccine [Vaccine] 2001 Dec 12; Vol. 20 (5-6), pp. 805-12. - Publication Year :
- 2001
-
Abstract
- Streptococcus pneumoniae is one of the most important human pathogens and improvement of the currently used polysaccharide vaccines is being pursued. We constructed DNA vaccine vectors containing either the full-length psaA (pneumococcal surface adhesin A) or a truncated pspA (pneumococcal surface protein A--pspA') gene. Both constructs showed transient expression of the antigens in vertebrate cells and induced significant antibody response to the pneumococcal antigens in BALB/c mice injected intramuscularly (i.m.). Fusion with an N-terminal cytoplasmatic SV40 T-antigen (CT-Ag), which was previously shown to stabilize poorly expressed antigens through association with Hsp73, also induced anti-PspA antibody response. The induction of antibodies with a low IgG1:IgG2a ratio and elevated gamma interferon (IFN-gamma) production by spleen cells elicited by DNA vaccination indicate preferential priming of Th1 immunity. Since induction of antibodies against both PsaA and PspA was previously shown to correlate with protection against fatal infection with S. pneumoniae and cell-mediated immune responses could contribute to protection, further evaluation of PsaA and PspA as antigens for a DNA vaccine against S. pneumoniae could be promising.
- Subjects :
- Adhesins, Bacterial
Animals
Antibodies, Bacterial biosynthesis
Bacterial Proteins genetics
Base Sequence
Carrier Proteins genetics
DNA, Bacterial genetics
Heat-Shock Proteins genetics
Humans
Immunity, Cellular
Interferon-gamma biosynthesis
Interleukin-4 biosynthesis
Lipoproteins genetics
Mice
Mice, Inbred BALB C
Pneumococcal Vaccines genetics
Pneumococcal Vaccines immunology
Streptococcus pneumoniae genetics
Th1 Cells immunology
Vaccines, DNA genetics
Vaccines, DNA immunology
Vaccines, DNA pharmacology
Bacterial Proteins immunology
Carrier Proteins immunology
Heat-Shock Proteins immunology
Lipoproteins immunology
Membrane Transport Proteins
Pneumococcal Vaccines pharmacology
Streptococcus pneumoniae immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0264-410X
- Volume :
- 20
- Issue :
- 5-6
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 11738744
- Full Text :
- https://doi.org/10.1016/s0264-410x(01)00395-4