Isolation of the major lethal toxin in the venom of Bungarus flaviceps.

Authors :: Khow O
Chanhome L
Omori-Satoh T
Sitprija V
Source :: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2002 Apr; Vol. 40 (4), pp. 463-9.
Publication Year :: 2002
Abstract: The major lethal toxin in the venom of Bungarus flaviceps has been isolated by ion-exchange chromatography, absorption chromatography and RP-HPLC with a 14-fold purification and an overall yield of 16.5% of the lethal toxicity contained in crude venom. Its sublethal dose (LD(50)) determined in mice weighing 18-20 g was 0.25 (0.19-0.32) microg per mouse. The lethal toxin was pure according to disc- and SDS-PAGE as well as gel HPLC. Its apparent molecular weight determined by SDS-PAGE was 29 kDa. It is a basic protein consisting of two polypeptide chains having apparent molecular weights of 17 and 8 kDa, respectively. The toxin has PLA activity but is free of ACE activity.

Subjects :: Animals
Bungarotoxins toxicity
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Electrophoresis, Polyacrylamide Gel
Injections, Intravenous
Lethal Dose 50
Mice
Molecular Weight
Peptides chemistry
Bungarotoxins chemistry
Bungarotoxins isolation & purification
Bungarus

Language :: English
ISSN :: 0041-0101
Volume :: 40
Issue :: 4
Database :: MEDLINE
Journal :: Toxicon : official journal of the International Society on Toxinology
Publication Type :: Academic Journal
Accession number :: 11738240
Full Text :: https://doi.org/10.1016/s0041-0101(01)00235-5