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Hepatocyte growth factor alters renal epithelial cell susceptibility to uropathogenic Escherichia coli.
- Source :
-
Journal of the American Society of Nephrology : JASN [J Am Soc Nephrol] 2001 Dec; Vol. 12 (12), pp. 2543-2553. - Publication Year :
- 2001
-
Abstract
- The urinary tract is frequently the source of Escherichia coli bacteremia. Bacteria from the urinary tract must cross an epithelial layer to enter the bloodstream. Hepatocyte growth factor (HGF) alters the polarity of Madin-Darby canine kidney (MDCK) epithelial cells. The role of cell polarity in determining renal epithelial resistance to Escherichia coli invasion is not well known. A model of polarized and HGF-treated MDCK epithelial cells grown on filters was used to study the role of epithelial cell polarity during the interaction of nonvirulent (XL1-Blue) and uropathogenic (J96) strains of Escherichia coli with renal epithelium. Basolateral exposure of MDCK cells to J96, but not XL1-Blue, resulted in loss of transepithelial resistance (TER), which was due to epithelial cytotoxicity and not degradation of epithelial junctional proteins by bacterial proteases. Apical exposure to both J96 and XL1-Blue did not alter TER. Pretreatment of polarized MDCK cell monolayers with HGF renders the cells sensitive to loss of TER and cytotoxicity by apical exposure to J96. Analysis by confocal microscopy demonstrated that HGF treatment of MDCK cell monolayers also greatly enhances adherence of J96 to the apical surface of the cell monolayer. These data demonstrate that the basolateral surface of polarized epithelia is more susceptible to J96 cytotoxicity. The data also support the hypothesis that processes that alter epithelial cell polarity increase sensitivity of epithelia to bacterial injury and adherence from the apical compartment.
- Subjects :
- Animals
Bacterial Translocation drug effects
Cadherins metabolism
Cell Communication physiology
Cell Line
Cell Membrane drug effects
Cell Membrane physiology
Cell Polarity drug effects
Cell Survival
Culture Media pharmacology
Disease Susceptibility
Dogs
Electric Impedance
Epithelial Cells drug effects
Epithelial Cells physiology
Escherichia coli physiology
Escherichia coli Infections physiopathology
Hydrolysis
Kidney cytology
Kidney physiopathology
Precipitin Tests
Escherichia coli Infections complications
Escherichia coli Infections etiology
Hepatocyte Growth Factor pharmacology
Kidney drug effects
Urologic Diseases microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1046-6673
- Volume :
- 12
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Journal of the American Society of Nephrology : JASN
- Publication Type :
- Academic Journal
- Accession number :
- 11729222
- Full Text :
- https://doi.org/10.1681/ASN.V12122543