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De novo regeneration of Scrophularia yoshimurae Yamazaki (Scrophulariaceae) and quantitative analysis of harpagoside, an iridoid glucoside, formed in aerial and underground parts of in vitro propagated and wild plants by HPLC.
- Source :
-
Biological & pharmaceutical bulletin [Biol Pharm Bull] 2001 Nov; Vol. 24 (11), pp. 1311-5. - Publication Year :
- 2001
-
Abstract
- A protocol for de novo regeneration and rapid micropropagation of Scrophularia yoshimurae (Scrophulariaceae) has been developed. Multiple shoot development was achieved by culturing the shoot-tip, leaf-base, stem-node and stem-internode explants on Murashige and Skoog (MS) medium supplemented with 4.44 microM N6-benzyladenine (BA) and 1.07 microM alpha-naphthaleneacetic acid (NAA). Stem-node and shoot-tip explants showed the highest response (100%) followed by stem-internode (74.4%) and leaf-base (7.7%) explants. The shoots were multiplied by subculturing on the same medium used for shoot induction. Shoots were rooted on growth regulator-free MS basal medium and the plantlets were transplanted to soil and acclimatized in the growth chamber. The content of harpagoside, a quantitatively predominant iridoid glycoside, in different plant material was determined by high performance liquid chromatography (HPLC). The analysis revealed that the content of harpagoside in the aerial and underground parts of S. yoshimurae was significantly higher than the marketed crude drug (underground parts of Scrophularia ningpoensis).
- Subjects :
- Chromatography, High Pressure Liquid methods
Culture Techniques methods
Glycosides physiology
Iridoids
Plant Extracts analysis
Plant Roots chemistry
Plant Roots physiology
Plant Shoots chemistry
Plant Shoots physiology
Glucosides analysis
Glycosides analysis
Pyrans analysis
Regeneration physiology
Scrophulariaceae chemistry
Scrophulariaceae physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0918-6158
- Volume :
- 24
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Biological & pharmaceutical bulletin
- Publication Type :
- Academic Journal
- Accession number :
- 11725970
- Full Text :
- https://doi.org/10.1248/bpb.24.1311