Back to Search
Start Over
DNA-based detection of prostate cancer in blood, urine, and ejaculates.
- Source :
-
Annals of the New York Academy of Sciences [Ann N Y Acad Sci] 2001 Sep; Vol. 945, pp. 51-8. - Publication Year :
- 2001
-
Abstract
- Background: Methylation-specific polymerase chain reaction (MSP) targeting promoter hypermethylation of the glutathione S transferase P1 gene (GSTP1), as the most frequent DNA alteration in prostatic carcinoma, was used for the molecular detection of cell-bound and cell-free prostate tumor DNA in various human bodily fluids.<br />Materials and Methods: We investigated GSTP1 promoter hypermethylation in DNA isolated from plasma, serum, nucleated blood cells, ejaculates, urines after prostate massage, and prostate tissue from 33 patients with prostate cancer and 26 control patients with benign prostatic hyperplasia (BPH). Using a viral DNA extraction kit specifically recommended for DNA isolation from urine samples, GSTP1 promoter hypermethylation in urine sediments after prostatic massage was investigated in a cohort of 29 patients with prostate cancer and 40 controls with BPH. Fluorescently labeled MSP products were analyzed on an automated gene sequencer.<br />Results: GSTP1 promoter hypermethylation was found in 90% of tumors (18 of 20), 72% of plasma or serum samples (23 of 32), 50% of ejaculates (4 of 8), and between 36% (4 of 11; normal DNA isolation kit) and 76% (22 of 29; viral kit) of exprimated urines from patients with prostate cancer. Also, MSP identified circulating tumor cells in 30% (10 out of 33) of prostate cancer patients. Except for one urine sample, GSTP1 promoter hypermethylation was not found in tissue and body fluids from patients with BPH.<br />Conclusion: GSTP1 promoter methylation analysis provides a highly specific tool for DNA-based diagnosis of prostate cancer in body fluids.
- Subjects :
- Aged
Base Sequence
Case-Control Studies
DNA Methylation
DNA Primers
DNA, Neoplasm blood
DNA, Neoplasm urine
Glutathione Transferase genetics
Humans
Male
Middle Aged
Prostatic Neoplasms blood
Prostatic Neoplasms genetics
Prostatic Neoplasms urine
Sensitivity and Specificity
DNA, Neoplasm analysis
Prostatic Neoplasms diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 0077-8923
- Volume :
- 945
- Database :
- MEDLINE
- Journal :
- Annals of the New York Academy of Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 11708494
- Full Text :
- https://doi.org/10.1111/j.1749-6632.2001.tb03863.x