Back to Search
Start Over
Cross-priming as a predominant mechanism for inducing CD8(+) T cell responses in gene gun DNA immunization.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2001 Nov 15; Vol. 167 (10), pp. 5549-57. - Publication Year :
- 2001
-
Abstract
- DNA immunization induces CD8(+) CTL responses by bone marrow-derived APCs, which are directly transfected with a plasmid DNA and/or acquire Ags from DNA-transfected non-APCs. To investigate the relative contribution of DNA-transfected APCs vs non-APCs to the initiation of CD8(+) T cell responses, we used tissue-specific promoter-directed gene expression and adoptive transfer systems in gene gun DNA immunization. In this study, we demonstrated that non-APC-specific gene expressions induced significant CD8(+) CTL and IFN-gamma-producing cells and Ab responses, whereas APC-specific gene expressions led to moderate CTL and IFN-gamma-producers, but no Ab responses. Interestingly, mice immunized with a non-APC-specific plasmid induced more rapid, vigorous, and prolonged proliferation of adoptively transferred Ag-specific CD8(+) T cells than APC-specific plasmid-immunized mice. In addition, the in vivo proliferative responses elicited by a non-APC-specific plasmid administration were dependent on TAP, but were independent of CD4(+) T cell help. Collectively, our results suggest that cross-priming, in which Ags expressed in non-APCs are taken up, processed, and presented by APCs, plays an important role in the initiation, magnitude, and maintenance of CD8(+) T cell responses in gene gun DNA immunization.
- Subjects :
- Adoptive Transfer
Animals
Cell Line
Cells, Cultured
Female
Histocompatibility Antigens Class II genetics
Humans
Immunization
Immunoglobulins biosynthesis
Interferon-gamma biosynthesis
Keratin-14
Keratins genetics
Lymphocyte Activation
Macrophage-1 Antigen genetics
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Ovalbumin immunology
Promoter Regions, Genetic
T-Lymphocytes, Cytotoxic transplantation
Viral Core Proteins immunology
Biolistics methods
T-Lymphocytes, Cytotoxic immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 167
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 11698425
- Full Text :
- https://doi.org/10.4049/jimmunol.167.10.5549