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Ultrastructural changes in calvarial osteoblast cytoskeleton after prostaglandin E2 administration in rats.
- Source :
-
Anatomia, histologia, embryologia [Anat Histol Embryol] 2001 Oct; Vol. 30 (5), pp. 301-7. - Publication Year :
- 2001
-
Abstract
- Recent studies on the cytoskeleton of osteoblasts have been made mainly using cultured cells. However, the morphology of cultured cells may be altered during subculture. Therefore, cytoskeletal changes of calvarial osteoblasts were investigated in situ by electron microscopy using the detergent perfusion method to preserve cell morphology as well as selectively observing the cytoskeleton in the presence of a high concentration of prostaglandin E2 (PGE2) in the calvarial periphery in rats. Rats were perfused with a mixture of Triton X-100 and glutaraldehyde, then the cytoskeleton was observed by transmission electron microscopy. In osteoblasts of the control group, thick bundles of microfilaments ran parallel to the long axis of the cells immediately below the cell membrane adjacent to the osteoid layer. In contrast, in the osteoblasts of the PGE2-administered group, the external morphology was changed to an asteroid or cubic shape, and thick bundles of microfilaments immediately below the cell membrane adjacent to the osteoid were not observed, although microfilament fibres, with a diameter of 5-6 nm, were observed in the cytoplasm.
- Subjects :
- Actin Cytoskeleton ultrastructure
Animals
Cells, Cultured
Cytoskeleton ultrastructure
Dinoprostone pharmacology
Male
Microscopy, Electron veterinary
Osteoblasts ultrastructure
Periosteum cytology
Rats
Rats, Wistar
Skull cytology
Cytoskeleton drug effects
Dinoprostone administration & dosage
Osteoblasts drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0340-2096
- Volume :
- 30
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Anatomia, histologia, embryologia
- Publication Type :
- Academic Journal
- Accession number :
- 11688741
- Full Text :
- https://doi.org/10.1046/j.1439-0264.2001.00340.x