Structure of nuclear pre-mRNA. VI. "Reversed repeats" in animal DNA and their hybridization with double-stranded regions of pre-mRNA.

Authors :: Church RB
Ryskov AP
Georgiev GP
Source :: Molecular biology [Mol Biol] 1975 Jan; Vol. 8 (4), pp. 503-9.
Publication Year :: 1975
Abstract: About 2% of mouse DNA reassociates at extremely low Cot values (10-7-10-6 mole-liter-1-sec). This DNA fraction was isolated with the aid of nuclease S1 and purified by chromatography on hydroxyapatite. The study of this fraction suggests that it has a structure of hairpin type. The DNA of the hairpins can hybridize with sequences forming the double-stranded regions in pre-mRNA. Probably at least some of the DNA of the hairpins, described as "reversed repeating sequences" of DNA, is transcribed with the formation of structures of hairpin type in pre-mRNA.

Subjects :: Animals
Carcinoma, Ehrlich Tumor
Chromatography
Deoxyribonucleases
Female
Formaldehyde
Hydroxyapatites
Kinetics
L Cells analysis
Mice
Nucleic Acid Conformation
Nucleic Acid Denaturation
Nucleic Acid Hybridization
Nucleic Acid Renaturation
RNA, Neoplasm analysis
Cell Nucleus analysis
DNA
RNA, Messenger analysis
Transcription, Genetic

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