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Dysfunctional endothelial nitric oxide biosynthesis in healthy smokers with impaired endothelium-dependent vasodilatation.
- Source :
-
Circulation [Circulation] 2001 Oct 16; Vol. 104 (16), pp. 1905-10. - Publication Year :
- 2001
-
Abstract
- Background: The mechanisms involved in the dysfunction of both endothelium-dependent vasodilatation (EDV) and NO biosynthesis related to smoking are unclear. In this study, EDV was assessed in healthy smokers and nonsmokers in vivo and, using serum from the same individuals, was related to the NO biosynthetic pathway in vitro.<br />Methods and Results: Flow-mediated EDV of the brachial artery was measured in 23 male patients (8 nonsmokers and 15 smokers). Serum was collected, added to confluent ( approximately 85%) monolayers of human umbilical vein endothelial cells (HUVECs), and incubated for 12 hours. Basal and substance P-stimulated NO production was measured. The HUVECs used for measuring basal NO production were lysed, and both endothelial NO synthase (eNOS) protein expression and eNOS activity were determined. EDV was lower in smokers compared with nonsmokers (P<0.001). HUVECs treated with serum from smokers compared with nonsmokers showed significantly lower basal (P<0.0001) and stimulated (P<0.02) NO production, higher eNOS expression (P<0.0001), but lower eNOS activity (P<0.004). There was a significant positive correlation between in vivo EDV and in vitro substance P-stimulated NO production (rho=0.57, P<0.01) and between basal NO production and eNOS activity (r=0.54, P<0.008) and a negative correlation between basal NO production and eNOS protein expression (r=-0.60, P<0.003). CONCLUIONS: This is the first study to combine an in vivo model with a near-physiological in vitro model to demonstrate an association between decreased NO production and reduced EDV. Cigarette smoking was associated with reduced EDV, NO generation, and eNOS activity in the presence of increased eNOS protein expression.
- Subjects :
- Adult
Blood Flow Velocity physiology
Blood Proteins pharmacology
Brachial Artery diagnostic imaging
Brachial Artery drug effects
Brachial Artery physiology
Cell Division drug effects
Cell Survival drug effects
Cells, Cultured
Cotinine blood
Endothelium, Vascular cytology
Endothelium, Vascular drug effects
Enzyme Activation drug effects
Humans
Linear Models
Male
Nitric Oxide Synthase metabolism
Nitric Oxide Synthase Type III
Nitroglycerin pharmacology
Substance P pharmacology
Ultrasonography
Vasodilation drug effects
Endothelium, Vascular physiology
Nitric Oxide biosynthesis
Smoking metabolism
Vasodilation physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1524-4539
- Volume :
- 104
- Issue :
- 16
- Database :
- MEDLINE
- Journal :
- Circulation
- Publication Type :
- Academic Journal
- Accession number :
- 11602492
- Full Text :
- https://doi.org/10.1161/hc4101.097525