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Mutations in the NS3 gene and 3'-NCR of Japanese encephalitis virus isolated from an unconventional ecosystem and implications for natural attenuation of the virus.
- Source :
-
Virology [Virology] 2001 Oct 10; Vol. 289 (1), pp. 129-36. - Publication Year :
- 2001
-
Abstract
- The T1P1 strain of Japanese encephalitis (JE) virus was recently isolated from paddy-free Liu-Chiu Islet in which natural JE antibody has been prevalent. In mouse neuroblastoma-derived Neuro-2a cells, T1P1 appeared significantly lower in virus productivity than another local isolate, CH1392. It implied that this new isolate possesses a characteristic viral replication pattern other than that of CH1392. T1P1 has also shown lower neurovirulence, which was reflected by a significantly higher LD(50) (2.44 x 10(6) PFU) than CH1392 (2.87 x 10(2) PFU). In comparison of the full-length RNA sequences between T1P1 and CH1392, a total of 7 nucleotides, including 1 in preM/M and 2 each in NS3, NS5, and the 3'-end noncoding region (NCR), appeared different. Of them, only the changes in NS3 (position 325, T for CH1392, A for T1P1; and position 364, G for CH1392 and A for T1P1) resulted in substitutions of deduced amino acids. There were two additional nucleotide changes appearing in the 3'-NCR. The amino acids 109 Phe and 122 Glu in NS3 of CH1392 were substituted by Ile and Lys, respectively, in T1P1. The unique growth properties and low virulence of T1P1 presented in this report were likely related to abnormal enzymatic activity due to mutations of the NS3 gene (especially position 364) and possibly to the mutations in the 3'-NCR. The natural attenuation of T1P1 that has been circulating in paddy-free Liu-Chiu Islet may account for the absence of clinical JE cases in past years.<br /> (Copyright 2001 Academic Press.)
- Subjects :
- Amino Acid Substitution
Animals
Animals, Suckling
Brain virology
Cell Line
Culex physiology
Ecosystem
Encephalitis Virus, Japanese genetics
Encephalitis Virus, Japanese growth & development
Encephalitis, Japanese epidemiology
Encephalitis, Japanese virology
Genome, Viral
Mice
Molecular Sequence Data
RNA Helicases
RNA, Viral biosynthesis
RNA, Viral genetics
Sequence Analysis, DNA
Serine Endopeptidases
Taiwan epidemiology
Tumor Cells, Cultured
Viral Nonstructural Proteins chemistry
Viral Plaque Assay
Virulence
Virus Replication
3' Untranslated Regions genetics
Culex virology
Encephalitis Virus, Japanese isolation & purification
Encephalitis Virus, Japanese pathogenicity
Mutation
Viral Nonstructural Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0042-6822
- Volume :
- 289
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 11601924
- Full Text :
- https://doi.org/10.1006/viro.2001.1033